Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Ussing chamber system for measuring intestinal permeability in health and disease
Sch Med, Wales; Newcastle Univ, England.
Sch Med, Wales.
Sch Med, Wales.
Sch Med, Wales.
Show others and affiliations
2019 (English)In: BMC Gastroenterology, ISSN 1471-230X, E-ISSN 1471-230X, Vol. 19, article id 98Article in journal (Refereed) Published
Abstract [en]

BackgroundThe relationship between intestinal epithelial integrity and the development of intestinal disease is of increasing interest. A reduction in mucosal integrity has been associated with ulcerative colitis, Crohns disease and potentially could have links with colorectal cancer development. The Ussing chamber system can be utilised as a valuable tool for measuring gut integrity. Here we describe step-by-step methodology required to measure intestinal permeability of both mouse and human colonic tissue samples ex vivo, using the latest equipment and software. This system can be modified to accommodate other tissues.MethodsAn Ussing chamber was constructed and adapted to support both mouse and human tissue to measure intestinal permeability, using paracellular flux and electrical measurements. Two mouse models of intestinal inflammation (dextran sodium sulphate treatment and T regulatory cell depletion using C57BL/6-FoxP3(DTR) mice) were used to validate the system along with human colonic biopsy samples.ResultsDistinct regional differences in permeability were consistently identified within mouse and healthy human colon. In particular, mice showed increased permeability in the mid colonic region. In humans the left colon is more permeable than the right. Furthermore, inflammatory conditions induced chemically or due to autoimmunity reduced intestinal integrity, validating the use of the system.ConclusionsThe Ussing chamber has been used for many years to measure barrier function. However, a clear and informative methods paper describing the setup of modern equipment and step-by-step procedure to measure mouse and human intestinal permeability isnt available. The Ussing chamber system methodology we describe provides such detail to guide investigation of gut integrity.

Place, publisher, year, edition, pages
BMC , 2019. Vol. 19, article id 98
Keywords [en]
Intestinal permeability; Colon; Transepithelial resistance; Paracellular flux
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:liu:diva-158972DOI: 10.1186/s12876-019-1002-4ISI: 000472479600006PubMedID: 31221083OAI: oai:DiVA.org:liu-158972DiVA, id: diva2:1338137
Note

Funding Agencies|Cancer Research Wales [506719]; Wellcome Trust [504182]; Cancer Research UK [C16731/A21200]; Cardiff University

Available from: 2019-07-19 Created: 2019-07-19 Last updated: 2019-11-11

Open Access in DiVA

fulltext(2318 kB)7 downloads
File information
File name FULLTEXT01.pdfFile size 2318 kBChecksum SHA-512
172378eacd16eddf1def2c9f15b97084cb5cd42f202907aa3bebe808d9d94dae5d1a9ec6b211eb70e59e1763fdaa6422b271cc72d52451d67448fb7083c5fba9
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Keita, Åsa
By organisation
Division of Surgery, Orthopedics and OncologyFaculty of Medicine and Health Sciences
In the same journal
BMC Gastroenterology
Gastroenterology and Hepatology

Search outside of DiVA

GoogleGoogle Scholar
Total: 7 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 9 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf