Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Mechanical characterization of squid giant axon membrane sheath and influence of the collagenous endoneurium on its properties
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Neuronic Engineering.ORCID iD: 0000-0001-6306-507x
Laboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Biomechanics section, KU Leuven, Leuven, Belgium.
Laboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Show others and affiliations
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, no 1, article id 8969Article in journal (Refereed) Published
Abstract [en]

To understand traumas to the nervous system, the relation between mechanical load and functional impairment needs to be explained. Cellular-level computational models are being used to capture the mechanism behind mechanically-induced injuries and possibly predict these events. However, uncertainties in the material properties used in computational models undermine the validity of their predictions. For this reason, in this study the squid giant axon was used as a model to provide a description of the axonal mechanical behavior in a large strain and high strain rate regime (ε=10%,ε⋅=1s−1), which is relevant for injury investigations. More importantly, squid giant axon membrane sheaths were isolated and tested under dynamic uniaxial tension and relaxation. From the lumen outward, the membrane sheath presents: an axolemma, a layer of Schwann cells followed by the basement membrane and a prominent layer of loose connective tissue consisting of fibroblasts and collagen. Our results highlight the load-bearing role of this enwrapping structure and provide a constitutive description that could in turn be used in computational models. Furthermore, tests performed on collagen-depleted membrane sheaths reveal both the substantial contribution of the endoneurium to the total sheath’s response and an interesting increase in material nonlinearity when the collagen in this connective layer is digested. All in all, our results provide useful insights for modelling the axonal mechanical response and in turn will lead to a better understanding of the relationship between mechanical insult and electrophysiological outcome.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, no 1, article id 8969
National Category
Medical Engineering Mechanical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-255034DOI: 10.1038/s41598-019-45446-yISI: 000472137700065Scopus ID: 2-s2.0-85067621816OAI: oai:DiVA.org:kth-255034DiVA, id: diva2:1337545
Note

QC 20190729

Available from: 2019-07-16 Created: 2019-07-16 Last updated: 2019-09-05Bibliographically approved

Open Access in DiVA

fulltext(4812 kB)31 downloads
File information
File name FULLTEXT01.pdfFile size 4812 kBChecksum SHA-512
e803704596fa0454a454cd8af25310521cf51efc6667b759d3a93b32602a093cf15ff6f9425063210c4b7e02511f36e29966b7b6e0edda08d04118651b608b5c
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Montanino, AnnaclauidaKleiven, Svein
By organisation
Neuronic Engineering
In the same journal
Scientific Reports
Medical EngineeringMechanical Engineering

Search outside of DiVA

GoogleGoogle Scholar
Total: 31 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 63 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf