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Tredimensionell bildrekonstruktion av konfokalmikroskopibilder av CdSe-kvantprickar i mänskliga endotelceller
KTH, School of Engineering Sciences (SCI).
KTH, School of Engineering Sciences (SCI).
2019 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Three-dimensional image reconstruction of micrographs from a laser scanning confocal microscope of CdSe-core quantum dots in human umbilical vein endothelial cells (English)
Abstract [sv]

Användandet av kvantprickar som fluorescenta markörer har vissa fördelar gentemot traditionella, organiska molekyler. Intensiteten av fluorescensen från kvantprickar är högre än från organiska molekyler för motsvarande mängd exitationsenergi, vilket gör att stråldosen, och därmed fototoxicitet i biologiska provet, kan sänkas. Kvantprickar har vanligtvis en diameter i storleksordningen några nanometer vilket är mindre än upplösningen hos konfokalmikroskop. Syftet med detta kandidatexamensarbete är att försöka få mer kontrast och detalj i mikroskopibilder tagna på endotelceller från mänskliga navelsträngar med tillsatta kvantprickar. En teknik som kallas för avfaltning med L1-normregularisering, kombinerat med Arnoldiiterationer, används i detta arbete. Avfaltningen resulterar i högre kontrast i vissa kritiska områden jämfört med i originalbilden. Den implementerade metoden kan vara ett alternativ till kommersiellt tillgängliga avfaltningsmetoder, men fortsatta studier krävs för att optimera hastigheten och kvalitén på avfaltningen.

Abstract [en]

Usage of Quantum Dots (QDs) for fluorescent labelling on biological samples has some favorable properties over traditional organic molecules. The intensity of the fluorescence from QDs is greater than that from some organic molecules, allowing lower excitation intensities which results in lower phototoxicity in the biological sample. QDs usually have a diameter in the the scale of a few nanometers, which is smaller than the resolution limit of confocal light microscopes. The aim of this thesis is to try to resolve more contrast and detail in micrographs of human umbilical vein endothelial cells stained with QDs. The method of choice here is deconvolution with L1-norm regularization combined with Arnoldi iterations for computational optimization. The resulting deconvolutions of fluorescence micrographs have higher constrast constrained to smaller spatial regions than in the original micrographs. We conclude that the regularization implemented here could possibly be a viable alternative to commercially available deconvolution methods, but more research is needed to optimize computation speed and accuracy.

Place, publisher, year, edition, pages
2019.
Series
TRITA-SCI-GRU ; 2019:134
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-254790OAI: oai:DiVA.org:kth-254790DiVA, id: diva2:1335351
Supervisors
Examiners
Available from: 2019-07-05 Created: 2019-07-05 Last updated: 2019-07-05Bibliographically approved

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