Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Antibiotic-Regulated Plasmid Copy Number Variation: A Driver of Antibiotic Resistance?
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. (Dan I. Andersson)
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Plasmids are small circular DNA molecules within bacterial cells that are separated from the bacterial chromosome and replicate independently. Also, they play a crucial role in the dissemination of antibiotic resistance genes among bacteria through horizontal gene transfer. They can be present in many copies within host cell, which is known as plasmid copy number. Plasmids can regulate their own copy number by different mechanisms. Additionally, the selective pressure can also play a pivotal role in determining plasmid copy number. The presence of antibiotics in the surrounding environment can drive variations of plasmid copy number. In this study, we examined plasmid copy number variations of multidrug resistance plasmids in presence of antibiotics by using EvaGreen® - based multiplexed digital droplet PCR. We could observe that cultures of Klebsiella pneumoniae and Escherichia coli harboring multidrug resistance plasmids grown in presence of sub-MIC concentrations of the antibiotics did not show high variations in plasmid copy numbers. On the other hand, mutants of K. pneumoniae selected for increased antibiotic resistance showed high increases in copy number of a multidrug-resistance plasmid.

Place, publisher, year, edition, pages
2019. , p. 26
Keywords [en]
antibiotic resistance, plasmid copy number, ddPCR
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-388523OAI: oai:DiVA.org:uu-388523DiVA, id: diva2:1333633
Educational program
Master Programme in Infection Biology
Presentation
2019-06-05, C8:321, BMC Husargatan 3 752 37, Uppsala, 09:45 (English)
Supervisors
Examiners
Available from: 2019-07-05 Created: 2019-07-01 Last updated: 2019-07-05Bibliographically approved

Open Access in DiVA

fulltext(1407 kB)15 downloads
File information
File name FULLTEXT01.pdfFile size 1407 kBChecksum SHA-512
66e99902dd15f1d6c181f50ee0c2747d74646ec7f2ff58b0ad59cfce27624e1f9cd9d41a7895284bbc369bc8a57517169d0454202e6b307d4da0f42470c8e641
Type fulltextMimetype application/pdf

By organisation
Department of Medical Biochemistry and Microbiology
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 15 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 83 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf