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Characterizing the cellular attachment receptor for Langat virus
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Section of Virology.ORCID iD: 0000-0002-2438-3080
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Section of Virology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Section of Virology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Section of Virology.ORCID iD: 0000-0002-7069-6678
2019 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 14, no 6, article id e0217359Article in journal (Refereed) Published
Abstract [en]

Tick-borne encephalitis infections have increased the last 30 years. The mortality associated to this viral infection is 0.5 to 30% with a risk of permanent neurological sequelae, however, no therapeutic is currently available. The first steps of virus-cell interaction, such as attachment and entry, are of importance to understand pathogenesis and tropism. Several molecules have been shown to interact with tick-borne encephalitis virus (TBEV) at the plasma membrane surface, yet, no studies have proven that these are specific entry receptors. In this study, we set out to characterize the cellular attachment receptor(s) for TBEV using the naturally attenuated member of the TBEV complex, Langat virus (LGTV), as a model. Inhibiting or cleaving different molecules from the surface of A549 cells, combined with inhibition assays using peptide extracts from high LGTV binding cells, revealed that LGTV attachment to host cells is dependent on plasma membrane proteins, but not on glycans or glycolipids, and suggested that LGTV might use different cellular attachment factors on different cell types. Based on this, we developed a transcriptomic approach to generate a list of candidate attachment and entry receptors. Our findings shed light on the first step of the flavivirus life-cycle and provide candidate receptors that might serve as a starting point for future functional studies to identify the specific attachment and/or entry receptor for LGTV and TBEV.

Place, publisher, year, edition, pages
Public Library Science , 2019. Vol. 14, no 6, article id e0217359
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-161188DOI: 10.1371/journal.pone.0217359ISI: 000470086200012PubMedID: 31163044OAI: oai:DiVA.org:umu-161188DiVA, id: diva2:1332621
Funder
Swedish Research Council, 349-2007-8673Available from: 2019-06-28 Created: 2019-06-28 Last updated: 2019-07-10Bibliographically approved

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Rodrigues, RaquelDanskog, KatarinaÖverby, Anna K.Arnberg, Niklas
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