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Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Biology. (Griese lab)
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR.

Place, publisher, year, edition, pages
2019. , p. 94
Series
UPTEC X ; 19 003
Keywords [en]
structural biology, cell culture, protein expression, genetic engineering, spectroscopy, X-ray crystallography, small angle X-ray scattering, SAXS, transcription factor, PerR, metal binding, antibiotics, Saccharopolyspora erythraea, S. erythraea, soil bacterium, actinobacteria, actinomycetes, oxidative stress, peroxide stress, structure determination, characterization, stabilization, peroxide sensor, iron sensor, manganese sensor, metalloprotein, metal ion homeostasis, metal ion, prokaryotic
Keywords [sv]
strukturbiologi, cellodling, proteinuttryck, genteknik, spektroskopi, röntgenkristallografi, småvinkel-röntgenspridning, SAXS, transkriptionsfaktor, PerR, metallbindande, antibiotika, Saccharopolyspora erythraea, S. erythraea, jordartsbakterie, Aktinobakterier, Actinobacteria, Actinomycetes, oxidativ stress, peroxid stress, strukturbestämning, karaktärisering, stabilisering, peroxidsensor, järnsensor, mangansensor, metalljonshomeostas, metalljoner, prokaryot
National Category
Structural Biology
Identifiers
URN: urn:nbn:se:uu:diva-387943OAI: oai:DiVA.org:uu-387943DiVA, id: diva2:1331130
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2019-06-28 Created: 2019-06-26 Last updated: 2019-06-28Bibliographically approved

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