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Mucus Architecture and Near-Surface Swimming Affect Distinct Salmonella Typhimurium Infection Patterns along the Murine Intestinal Tract
Swiss Fed Inst Technol, Inst Microbiol, CH-8093 Zurich, Switzerland.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Swiss Fed Inst Technol, Inst Microbiol, CH-8093 Zurich, Switzerland.ORCID iD: 0000-0002-8355-0803
Univ Gothenburg, Dept Med Biochem, S-40530 Gothenburg, Sweden.
Swiss Fed Inst Technol, Inst Microbiol, CH-8093 Zurich, Switzerland.
2019 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 27, no 9, p. 2665-26788.e1–e3Article in journal (Refereed) Published
Abstract [en]

Mucus separates gut-luminal microbes from the tissue. It is unclear how pathogens like Salmonella Typhimurium (S.Tm) can overcome this obstacle. Using live microscopy, we monitored S.Tm interactions with native murine gut explants and studied how mucus affects the infection. A dense inner mucus layer covers the distal colon tissue, limiting direct tissue access. S.Tm performs near-surface swimming on this mucus layer, which allows probing for colon mucus heterogeneities, but can also entrap the bacterium in the dense inner colon mucus layer. In the cecum, dense mucus fills only the bottom of the intestinal crypts, leaving the epithelium between crypts unshielded and prone to access by motile and non-motile bacteria alike. This explains why the cecum is highly infection permissive and represents the primary site of S.Tm enterocolitis in the streptomycin mouse model. Our findings highlight the importance of mucus in intestinal defense and homeostasis.

Place, publisher, year, edition, pages
CELL PRESS , 2019. Vol. 27, no 9, p. 2665-26788.e1–e3
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-386448DOI: 10.1016/j.celrep.2019.04.106ISI: 000469216500013PubMedID: 31141690OAI: oai:DiVA.org:uu-386448DiVA, id: diva2:1330909
Funder
EU, European Research Council, 694181Knut and Alice Wallenberg FoundationSwedish Research Council, 2012-262 2015-00635Swedish Nutrition Foundation (SNF), 310030_153074; 310030B_173338/1; Sinergia CRSII_154414/1Available from: 2019-06-26 Created: 2019-06-26 Last updated: 2019-06-26Bibliographically approved

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