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WAKMAR2, a Long Noncoding RNA Downregulated in Human Chronic Wounds, Modulates Keratinocyte Motility and Production of Inflammatory Chemokines
Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden.
Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden.
Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden.
Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden.
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2019 (English)In: Journal of Investigative Dermatology, ISSN 0022-202X, E-ISSN 1523-1747, Vol. 139, no 6, p. 1373-1384Article in journal (Refereed) Published
Abstract [en]

Chronic wounds represent a major and growing health and economic burden worldwide. A better understanding of molecular mechanisms of normal as well as impaired wound healing is needed to develop effective treatment. Herein we studied the potential role of long noncoding RNA LOC100130476 in skin wound repair. LOC100130476 is an RNA polymerase IIeencoded polyadenylated transcript present in both cytoplasm and nucleus. We found that its expression was lower in wound-edge keratinocytes of human chronic wounds compared to normal wounds of healthy donors and intact skin. In cultured keratinocytes, LOC100130476 expression was induced by TGF-beta signaling. By reducing LOC100130476 expression with antisense oligos or activating its transcription with CRISPR/Cas9 Synergistic Activation Mediator system, we showed that LOC100130476 restricted the production of inflammatory chemokines by keratinocytes, while enhancing cell migration. In line with this, knockdown of LOC100130476 impaired re-epithelization of human ex vivo wounds. Based on these results, we named LOC100130476 wound and keratinocyte migration-associated long noncoding RNA 2 (WAKMAR2). Moreover, we identified a molecular network that may mediate the biological function of WAKMAR2 in keratinocytes using microarray. In summary, our data suggest that WAKMAR2 is an important regulator of skin wound healing and its deficiency may contribute to the pathogenesis of chronic wounds.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC , 2019. Vol. 139, no 6, p. 1373-1384
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Dermatology and Venereal Diseases
Identifiers
URN: urn:nbn:se:uu:diva-386443DOI: 10.1016/j.jid.2018.11.033ISI: 000468701800030PubMedID: 30594489OAI: oai:DiVA.org:uu-386443DiVA, id: diva2:1330456
Funder
Swedish Research Council, 2013-03085; 2015-06246; 2016-02051; 2016-01586Ragnar Söderbergs stiftelseLars Hierta Memorial FoundationStiftelsen Olle Engkvist ByggmästareTore Nilsons Stiftelse för medicinsk forskningThe Karolinska Institutet's Research FoundationStockholm County CouncilSwedish Society for Medical Research (SSMF)Available from: 2019-06-25 Created: 2019-06-25 Last updated: 2019-06-25Bibliographically approved

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