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Stereochemical identification of glucans by a donor-acceptor-donor conjugated pentamer enables multi-carbohydrate anatomical mapping in plant tissues
Karolinska Inst, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
Karolinska Inst, Sweden.
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2019 (English)In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 26, no 7, p. 4253-4264Article in journal (Refereed) Published
Abstract [en]

Optotracing is a novel method for analytical imaging of carbohydrates in plant and microbial tissues. This optical method applies structure-responsive oligothiophenes as molecular fluorophores emitting unique optical signatures when bound to polysaccharides. Herein, we apply Carbotrace680, a short length anionic oligothiophene with a central heterocyclic benzodithiazole (BTD) motif, to probe for different glucans. The donor-acceptor-donor type electronic structure of Carbotrace680 provides improved spectral properties compared to oligothiophenes due to the possibility of intramolecular charge-transfer transition to the BTD motif. This enables differentiation of glucans based on the glycosidic linkage stereochemistry. Thus -configured starch is readily differentiated from -configured cellulose. The versatility of optotracing is demonstrated by dynamic monitoring of thermo-induced starch remodelling, shown in parallel by spectrophotometry and microscopy of starch granules. Imaging of Carbotrace680 bound to multiple glucans in plant tissues provided direct identification of their physical locations, revealing the spatial relationship between structural (cellulose) and storage (starch) glucans at sub-cellular scale. Our work forms the basis for the development of superior optotracers for sensitive detection of polysaccharides. Our non-destructive method for anatomical mapping of glucans in biomass will serve as an enabling technology for developments towards efficient use of plant-derived materials and biomass. [GRAPHICS] .

Place, publisher, year, edition, pages
SPRINGER , 2019. Vol. 26, no 7, p. 4253-4264
Keywords [en]
Cellulose; Starch; Glucose polysaccharides; Optotracing; Non-disruptive carbohydrate analysis
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URN: urn:nbn:se:liu:diva-157546DOI: 10.1007/s10570-019-02381-5ISI: 000465576000002OAI: oai:DiVA.org:liu-157546DiVA, id: diva2:1328668
Note

Funding Agencies|Carl Bennet AB; Erling-Persson Family Foundation; Swedish Foundation for Strategic Research; Swedish Research Council

Available from: 2019-06-22 Created: 2019-06-22 Last updated: 2019-11-04

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