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Enkel UV-absorptionsfotometer för absorbansmätning i medicinska prover
Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics. Umeå University, Faculty of Medicine, Department of Radiation Sciences.
2019 (Swedish)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesisAlternative title
Simple UV-absorption photometer for absorbance measurement in medical samples (English)
Abstract [sv]

Inom laboratoriemedicin finns utrustning som spektralfotometern som kan mäta absorption i en lösning för att bestämma koncentrationen av proteiner, aminosyror och liknande. Detta är en ofta en dyr och svårtillgänglig utrustning. Avdelningen Medicinsk Teknik - Forskning och Utveckling (MT-FoU) vid Norrlands Universitetssjukhus har fått förfrågan om att undersöka möjligheterna till att konstruera en enkel och billig UV-absorptionsfotometer. Målet var att ta fram en prototyp av en UV-absorptionsfotmoter för att kunna mäta koncentrationen av SAICA-r och S-Ado vilket förekommer i urinet vid sjukdomen Adenolosuccinat lyase deficiency. För detta examensarbete valdes Adenosine triphosphate (ATP) som mätsubstans, eftersom ATP är ett stabilt protein som är lösligt i vatten och som tål att frysa och tinas. ATP har dessutom ett absorbansspektra som ligger väldigt nära SAICA-r och S-Ado. En fungeradeprototyp av UV-absorptionsfotometern har tagits fram. Prototypen påvisar en god linjäritet (R2 = 0.9956) för koncentrationer mellan 0-50 mg/L samt en hög noggrannhet för absorbans σ=0.0006. Med dessa resultat bedöms prototyp vara kapabel till att mäta halten SAICAr och S-Ado.

Abstract [en]

In laboratory medicine there is equipment such as the spectralphotometer that can measure absorption in a solution to determine the concentration of proteins, amino acids and the like. This is an often expensive equipment and often not readily available. The Department of Biomedical Engineering - Research and Development (BE-RD) at University hospital of Umeå has been asked to investigate the possibilities of constructing a simple and cheap UV-absorption photometer. The aim of this study was to produce a functioning prototype of a UV-absorption photometer that would be able to measure the concentration of SAICA-r and S-Ado which occurs in the urine of the rare disease Adenolosuccinate lyase deficiency (ASL). For this bachelor thesis, adenosine triphosphate (ATP) was chosen as measuring substance, since ATP is a stable protein which is soluble in water and which can be frozen and defrosted. It also has an absorbance spectra which is very close to SAICA-r and S-Ado. A functioning prototype of the UV-absorption photometer has been developed. The prototype demonstrates a good linearity(R2 = 0.9956) for concentrations between 0-50 mg/L and a high accuracy for absorbance σ=0.0006. With these results, the prototype is judged to be capable of measuring the content of SAICAr and S-Ado in a urine sample.

Place, publisher, year, edition, pages
2019. , p. 31
Keywords [en]
UV-absorption photometry
Keywords [sv]
UV-absorptionsfotometer
National Category
Electrical Engineering, Electronic Engineering, Information Engineering Medical Engineering
Identifiers
URN: urn:nbn:se:umu:diva-160351OAI: oai:DiVA.org:umu-160351DiVA, id: diva2:1326135
External cooperation
Norrlands universitetssjukhus (NUS) avd Medicinsk Teknik - Forskning och Utveckling (MT-FoU)
Subject / course
Elektronik
Educational program
Bachelor of Science Programme in Electronic and Computer Engeneering/ Medical engineering
Presentation
2019-06-04, TC335, 10:00 (Swedish)
Supervisors
Examiners
Available from: 2019-06-18 Created: 2019-06-17 Last updated: 2019-06-18Bibliographically approved

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Department of Applied Physics and ElectronicsDepartment of Radiation Sciences
Electrical Engineering, Electronic Engineering, Information EngineeringMedical Engineering

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