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Fish Hybridization Leads to Uncertainty Regarding Ciguatera Fish Poisoning Risk; Confirmation of Hybridization and Ciguatoxin Accumulation with Implications for Stakeholders
US FDA, Div Seafood Sci & Technol, Dauphin Isl, AL 36528 USA;German Fed Inst Risk Assessment, Contaminants, Max Dohrn Str 8-10, D-10589 Berlin, Germany;Univ Napoli Federico II, Sch Med & Surg, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy.
US FDA, Off Regulatory Sci, College Pk, MD 20740 USA.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. US FDA, Div Seafood Sci & Technol, Dauphin Isl, AL 36528 USA.
US FDA, Off Regulatory Sci, College Pk, MD 20740 USA.
2019 (English)In: Journal of Marine Science and Engineering, E-ISSN 2077-1312, Vol. 7, no 4, article id 105Article in journal (Refereed) Published
Abstract [en]

Globally, ciguatera fish poisoning (CFP) avoidance efforts rely primarily on local knowledge of the fish being consumed, its collection location, and association with illnesses. In 2016, several fish that appeared to be hybrids between a local commercially prized species, Ocyurus chrysurus, and a regionally prohibited species Lutjanus apodus (due to CFP concerns), were caught nearshore in United States Virgin Islands waters, leading to confusion regarding the safety of consuming the fish. The hybrid status of the fish was verified as O. chrysurus (male) x L. apodus (female) by comparing two sets of gene sequences (mitochondrial CO1 and nuclear S7). Using an in vitro mouse neuroblastoma (N2a) assay, one of the hybrid fish exhibited a composite cytotoxicity of 0.038 ppb Caribbean ciguatoxin-1 (C-CTX-1) equivalents (Eq.); a concentration below the US Food and Drug Administration (FDA) guidance level for safety in fish products for CFP (0.1 ppb C-CTX-1 Eq.) but approximately 2x above the maximum described in the commercially prized parent species (0.019 ppb C-CTX-1 Eq./g). C-CTX-1 was confirmed in the hybrid sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The second hybrid fish tested negative for CTXs. This research confirms hybridization between two species with contrasting commercial statuses, discusses CTX accumulation implications for hybridization, and provides a methodology for future studies into novel CFP vectors, with the goal of providing critical information for fishermen and consumers regarding CFP risk management.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 7, no 4, article id 105
Keywords [en]
ciguatoxin, ciguatera poisoning, hybrid, DNA-based species identification, LC-MS, N2a assay, seafood poisoning
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-385578DOI: 10.3390/jmse7040105ISI: 000467498000024OAI: oai:DiVA.org:uu-385578DiVA, id: diva2:1325926
Available from: 2019-06-17 Created: 2019-06-17 Last updated: 2019-06-17Bibliographically approved

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