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Dynamin inhibitors impair platelet-derived growth factor beta-receptor dimerization and signaling
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2019 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 380, no 1, p. 69-79Article in journal (Refereed) Published
Abstract [en]

The role of plasma membrane composition and dynamics in the activation process of receptor tyrosine kinases (RTKs) is still poorly understood. In this study we have investigated how signaling via the RTK, platelet-derived growth factor beta-receptor (PDGFR-beta) is affected by Dynasore or Dyngo-4a, which are commonly used dynamin inhibitors. PDGFR-beta preferentially internalizes via clathrin-coated pits and in this pathway, Dynamin II has a major role in the formation and release of vesicles from the plasma membrane by performing the membrane scission. We have found that dynamin inhibitors impedes the activation of PDGFR-beta by impairing ligand-induced dimerization of the receptor monomers, which leads to a subsequent lack of phosphorylation and activation both of receptors and downstream effectors, such as ERK1/2 and AKT. In contrast, dynamin inhibitors did not affect epidermal growth factor receptor (EGFR) dimerization and phosphorylation. Our findings suggest that there is a link between plasma membrane dynamics and PDGFR-beta activation, and that this link is not shared with the epidermal growth factor receptor.

Place, publisher, year, edition, pages
ELSEVIER INC , 2019. Vol. 380, no 1, p. 69-79
Keywords [en]
RTK signaling, EGFR, PDGFR-beta, Dynasore, Dyngo, Dimerization
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-384986DOI: 10.1016/j.yexcr.2019.04.004ISI: 000468124700008PubMedID: 30970237OAI: oai:DiVA.org:uu-384986DiVA, id: diva2:1324952
Funder
Swedish Foundation for Strategic Research Swedish Research CouncilSwedish Cancer Society, CAN 2018/425Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-14Bibliographically approved

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Heldin, JohanRubin Sander, MarieLeino, MattiasLennartsson, JohanSöderberg, Ola
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