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Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa
Univ Murcia, Spain.
Univ Murcia, Spain; Univ Girona, Spain.
Univ Murcia, Spain; Yangzhou Univ, Peoples R China.
Univ Murcia, Spain.
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2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 7, article id 1791Article in journal (Refereed) Published
Abstract [en]

Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT spermatozoa were analyzed using a LC-ESI-MS/MS-based Sequential Window Acquisition of All Theoretical Spectra approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein-protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 20, no 7, article id 1791
Keywords [en]
epididymis; ejaculate; spermatozoa; cryopreservation; proteomics; porcine
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-157217DOI: 10.3390/ijms20071791ISI: 000464971200010PubMedID: 30978930OAI: oai:DiVA.org:liu-157217DiVA, id: diva2:1324383
Note

Funding Agencies|MINECO (Spain); FEDER funds (EU) [AGL2015-69738-R]; Seneca Foundation Murcia, Spain [19892/GERM-15]; FORSS [745971]; Swedish Research Council FORMAS, Stockholm, Sweden [2017-00946]; Seneca Foundation (Murcia, Spain); China Scholarship Council

Available from: 2019-06-13 Created: 2019-06-13 Last updated: 2019-10-31

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