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The time course of calprotectin liberation from human neutrophil granulocytes after Escherichia coli and endotoxin challenge
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Hedenstierna laboratory.ORCID iD: 0000-0002-1976-4129
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infection medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.ORCID iD: 0000-0002-3563-6450
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2019 (English)In: Innate Immunity, ISSN 1753-4259, E-ISSN 1753-4267, Vol. 25, no 6, p. 369-373Article in journal (Refereed) Published
Abstract [en]

Plasma calprotectin has previously been reported as a biomarker for sepsis. The aim of the present study was to elucidate the kinetics of calprotectin release from neutrophils exposed to Escherichia coli and endotoxin. Whole blood samples were exposed to E. coli bacteria or endotoxin in vitro. Blood samples were collected after 0, 1, 2, 3 and 4 h and plasma calprotectin was analysed by particle enhanced turbidimetric immunoassay while TNF-α, IL-6, neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) were analyzed by ELISA. When neutrophils were exposed to either E. coli or endotoxin, calprotectin levels began to increase within a couple of hours after the challenge. Calprotectin increases early in response to bacterial challenge. Given the logistic advantages of the calprotectin analysis, this may be of interest for early diagnosis of bacterial infections.

Place, publisher, year, edition, pages
2019. Vol. 25, no 6, p. 369-373
Keywords [en]
Sepsis, biomarkers, calprotectin, neutrophil gelatinase-associated lipocalin, systemic inflammatory response syndrome
National Category
Infectious Medicine Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-385063DOI: 10.1177/1753425919848476ISI: 000475346500006PubMedID: 31109223OAI: oai:DiVA.org:uu-385063DiVA, id: diva2:1322479
Available from: 2019-06-10 Created: 2019-06-10 Last updated: 2019-08-16Bibliographically approved

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Lipcsey, MiklósHanslin, KatjaSmekal, DavidLarsson, Anders
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