Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Role of pannexin-1 in the cellular uptake, release and hydrolysis of anandamide by T84 colon cancer cells
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.ORCID iD: 0000-0002-6884-4774
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.ORCID iD: 0000-0002-6658-7874
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 7622Article in journal (Refereed) Published
Abstract [en]

The large pore ion channel pannexin-1 (Panx1) has been reported to play a role in the cellular uptake and release of anandamide (AEA) in the hippocampus. It is not known whether this is a general mechanism or limited to the hippocampus. We have investigated this pharmacologically using T84 colon cancer cells. The cells expressed Panx1 at the mRNA level, and released ATP in a manner that could be reduced by treatment with the Panx1 inhibitors carbenoxolone and mefloquine and the Panxl substrate SR101. However, no significant effects of these compounds upon the uptake or hydrolysis of exogenously applied AEA was seen. Uptake by T84 cells of the other main endocannabinoid 2-arachidonoylglycerol and the AEA homologue palmitoylethanolamide was similarly not affected by carbenoxolone or mefloquine. Total release of tritium from [H-3]AEA-prelabelled T84 cells over 10 min was increased, rather than inhibited by carbenoxolone and mefloquine. Finally, AEA uptake by PC3 prostate cancer and SH-SY5Y neuroblastoma cells, which express functional Panx1 channels, was not inhibited by carbenoxolone. Thus, in contrast to the hippocampus, Panx1 does not appear to play a role in AEA uptake and release from the cells studied under the conditions used.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, article id 7622
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:umu:diva-159858DOI: 10.1038/s41598-019-44057-xISI: 000468281500052PubMedID: 31110238OAI: oai:DiVA.org:umu-159858DiVA, id: diva2:1322191
Funder
Swedish Research Council, 12158Available from: 2019-06-10 Created: 2019-06-10 Last updated: 2019-06-10Bibliographically approved

Open Access in DiVA

fulltext(5390 kB)27 downloads
File information
File name FULLTEXT01.pdfFile size 5390 kBChecksum SHA-512
223e1c1e74fbb1cf82fbf8b567a43f10f6ebbe0852e8a5a4a234d29ffbda5792cdfad4a6e61befa1eaea7fc541268987b3245d08bac6a2b4f6d59d5e581208e8
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Alhouayek, MireilleGilthorpe, Jonathan D.Fowler, Christopher J
By organisation
Department of Pharmacology and Clinical NeurosciencePharmacology
In the same journal
Scientific Reports
Pharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
Total: 27 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 247 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf