Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gene expression profile of extraocular muscles following resection strabismus surgery
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.ORCID iD: 0000-0002-7240-6515
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Show others and affiliations
2019 (English)In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 182, p. 182-193Article in journal (Refereed) Published
Abstract [en]

This paper aims to identify key biological processes triggered by resection surgery in the extraocular muscles (EOMs) of a rabbit model of strabismus surgery by studying changes in gene expression. Resection surgery was performed in the superior rectus of 16 rabbits and a group of non-operated rabbits served as control. Muscle samples were collected from groups of four animals 1, 2, 4 and 6 weeks after surgery and processed for RNA-sequencing and immunohistochemistry. We identified a total of 164; 136; 64 and 12 differentially expressed genes 1, 2, 4 and 6 weeks after surgery. Gene Ontology enrichment analysis revealed that differentially expressed genes were involved in biological pathways related to metabolism, response to stimulus mainly related with regulation of immune response, cell cycle and extracellular matrix. A complementary pathway analysis and network analysis performed with Ingenuity Pathway Analysis tool corroborated and completed these findings. Collagen I, fibronectin and versican, evaluated by immunofluorescence, showed that changes at the gene expression level resulted in variation at the protein level. Tenascin-C staining in resected muscles demonstrated the formation of new tendon and myotendinous junctions. These data provide new insights about the biological response of the EOMs to resection surgery and may form the basis for future strategies to improve the outcome of strabismus surgery.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 182, p. 182-193
Keywords [en]
Extraocular muscles, Strabismus, Gene expression, Resection surgery
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:umu:diva-159869DOI: 10.1016/j.exer.2019.03.022ISI: 000468258300021PubMedID: 30953624OAI: oai:DiVA.org:umu-159869DiVA, id: diva2:1322090
Funder
Swedish Research Council, 2018-02401Västerbotten County CouncilAvailable from: 2019-06-10 Created: 2019-06-10 Last updated: 2019-06-10Bibliographically approved

Open Access in DiVA

fulltext(3956 kB)26 downloads
File information
File name FULLTEXT01.pdfFile size 3956 kBChecksum SHA-512
234974e3e734c834cb430d21d332d83c2fa7719b2fda690b0ad82931670c9690a81b5c4167f3bbc52026e5136d175732af889ff725e3ff942b34744d1abe9d12
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rodríguez, Maria AngelsSandgren Hochhard, KarinVicente, AndréLiu, Jing-XiaDomellöf, Fatima Pedrosa
By organisation
OphthalmologyAnatomy
In the same journal
Experimental Eye Research
Ophthalmology

Search outside of DiVA

GoogleGoogle Scholar
Total: 26 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 86 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf