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Functional Expression of Gloeobacter Rhodopsin in PSI-Less Synechocystis sp. PCC6803
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2019 (English)In: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 7, article id 67Article in journal (Refereed) Published
Abstract [en]

The approach of providing an oxygenic photosynthetic organism with a cyclic electron transfer system, i.e., a far-red light-driven proton pump, is widely proposed to maximize photosynthetic efficiency via expanding the absorption spectrum of photosynthetically active radiation. As a first step in this approach, Gloeobacter rhodopsin was expressed in a PSI-deletion strain of Synechocystis sp. PCC6803. Functional expression of Gloeobacter rhodopsin, in contrast to Proteorhodopsin, did not stimulate the rate of photoheterotrophic growth of this Synechocystis strain, analyzed with growth rate measurements and competition experiments. Nevertheless, analysis of oxygen uptake and-production rates of the Gloeobacter rhodopsin-expressing strains, relative to the 1 PSI control strain, confirm that the proton-pumping Gloeobacter rhodopsin provides the cells with additional capacity to generate proton motive force. Significantly, expression of the Gloeobacter rhodopsin did modulate levels of pigment formation in the transgenic strain.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019. Vol. 7, article id 67
Keywords [en]
retinal-based proton pump, PSI-deletion Synechocystis, growth stimulation, carotenoid metabolism, oxygen evolution
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-158747DOI: 10.3389/fbioe.2019.00067ISI: 000463016900002PubMedID: 30984754OAI: oai:DiVA.org:umu-158747DiVA, id: diva2:1315935
Available from: 2019-05-15 Created: 2019-05-15 Last updated: 2019-05-15Bibliographically approved

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Cheregi, OtiliaFunk, Christiane
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