Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology.
Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA.
Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA;Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA;Harvard Univ, Dept Phys, Cambridge, MA 02138 USA.
Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA.
Show others and affiliations
2019 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 10, article id 1720Article in journal (Refereed) Published
Abstract [en]

ATP-dependent chromatin remodelling enzymes (remodellers) regulate DNA accessibility in eukaryotic genomes. Many remodellers reposition (slide) nucleosomes, however, how DNA is propagated around the histone octamer during this process is unclear. Here we examine the real-time coordination of remodeller-induced DNA movements on both sides of the nucleosome using three-colour single-molecule FRET. During sliding by Chd1 and SNF2h remodellers, DNA is shifted discontinuously, with movement of entry-side DNA preceding that of exit-side DNA. The temporal delay between these movements implies a single ratelimiting step dependent on ATP binding and transient absorption or buffering of at least one base pair. High-resolution cross-linking experiments show that sliding can be achieved by buffering as few as 3 bp between entry and exit sides of the nucleosome. We propose that DNA buffering ensures nucleosome stability during ATP-dependent remodelling, and provides a means for communication between remodellers acting on opposite sides of the nucleosome.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 10, article id 1720
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-382551DOI: 10.1038/s41467-019-09657-1ISI: 000464338100031PubMedID: 30979890OAI: oai:DiVA.org:uu-382551DiVA, id: diva2:1314851
Funder
EU, European Research CouncilSwedish Research Council, VR 2015-04568Knut and Alice Wallenberg Foundation, KAW/WAF 2014.0183
Note

These authors contributed equally: Anton Sabantsev, Robert F. Levendosky.

Available from: 2019-05-10 Created: 2019-05-10 Last updated: 2019-05-10Bibliographically approved

Open Access in DiVA

fulltext(1542 kB)50 downloads
File information
File name FULLTEXT01.pdfFile size 1542 kBChecksum SHA-512
6957bdfc8cfa14dc1625f5c6fb58af79d0c595f5835c2641df7e1137b244af2079a7ae999aa0a5ec3ed78f212ba0c3af55a7a50988d9344cf008e0f5fe9c9b47
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Sabantsev, AntonDeindl, Sebastian
By organisation
Science for Life Laboratory, SciLifeLabMolecular Systems Biology
In the same journal
Nature Communications
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 50 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 85 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf