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A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius
Stockholm Univ, Dept Mol Biosci, Wenner Gren Inst, Svante Arrhenius V 20C, SE-10691 Stockholm, Sweden.
Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Pl Laan 2, B-1050 Brussels, Belgium.
Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Pl Laan 2, B-1050 Brussels, Belgium.
Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, Pl Laan 2, B-1050 Brussels, Belgium.
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2019 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 10, article id 1542Article in journal (Refereed) Published
Abstract [en]

Fatty acid metabolism and its regulation are known to play important roles in bacteria and eukaryotes. By contrast, although certain archaea appear to metabolize fatty acids, the regulation of the underlying pathways in these organisms remains unclear. Here, we show that a TetR-family transcriptional regulator (FadR(sa)) is involved in regulation of fatty acid metabolism in the crenarchaeon Sulfolobus acidocaldarius. Functional and structural analyses show that FadR(sa) binds to DNA at semi-palindromic recognition sites in two distinct stoichiometric binding modes depending on the operator sequence. Genome-wide transcriptomic and chromatin immunoprecipitation analyses demonstrate that the protein binds to only four genomic sites, acting as a repressor of a 30-kb gene cluster comprising 23 open reading frames encoding lipases and beta-oxidation enzymes. Fatty acyl-CoA molecules cause dissociation of FadR(sa) binding by inducing conformational changes in the protein. Our results indicate that, despite its similarity in overall structure to bacterial TetR-family FadR regulators, FadR(sa) displays a different acyl-CoA binding mode and a distinct regulatory mechanism.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 10, article id 1542
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Microbiology Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-382385DOI: 10.1038/s41467-019-09479-1ISI: 000463313200018PubMedID: 30948713OAI: oai:DiVA.org:uu-382385DiVA, id: diva2:1307205
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Swedish Research Council, 621-2013-4685Available from: 2019-04-26 Created: 2019-04-26 Last updated: 2019-04-26Bibliographically approved

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