Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
Umeå Univ, Dept Clin Microbiol, Virol, Umeå, Sweden.
Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden.
Umeå Univ, Dept Clin Microbiol, Umeå, Sweden.
Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden.
Show others and affiliations
2019 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 19, article id 164Article in journal (Refereed) Published
Abstract [en]

Background: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera.

Methods: Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples.

Results: AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900–0.979) and 0.989 (95% CI 0.969–0.996) in the biobank and clinical samples, respectively.

Conclusion: The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.

Place, publisher, year, edition, pages
2019. Vol. 19, article id 164
Keywords [en]
Herpes simplex, IgG, Avidity, ELISA, Primary infection, Reactivated infection
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:uu:diva-378996DOI: 10.1186/s12879-019-3769-xISI: 000459030400003PubMedID: 30764767OAI: oai:DiVA.org:uu-378996DiVA, id: diva2:1297235
Available from: 2019-03-19 Created: 2019-03-19 Last updated: 2019-03-19Bibliographically approved

Open Access in DiVA

fulltext(662 kB)11 downloads
File information
File name FULLTEXT01.pdfFile size 662 kBChecksum SHA-512
11d7871234a2e41123e8d6d20fd70010d6dca0beb539dbb9baf58ae6556cec084dbb80f507c601081b4a30e0f501901a7ae76ae80b92976b8162633227ce0977
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Weidung, Bodil
By organisation
Geriatrics
In the same journal
BMC Infectious Diseases
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 11 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 17 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf