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miR-30e-5p as predictor of generalization in ocular myasthenia gravis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Clinical Neurophysiology.
Nottingham Univ Hosp NHS Trust, Dept Neurol, Queens Med Ctr, Nottingham, Notts, England.
Moorfields Eye Hosp NHS Fdn Trust, Dept Neuroophthalmol, London, England.
Nottingham Univ Hosp NHS Trust, Dept Neurol, Queens Med Ctr, Nottingham, Notts, England.
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2019 (English)In: Annals of clinical and translational neurology, E-ISSN 2328-9503, Vol. 6, no 2, p. 243-251Article in journal (Refereed) Published
Abstract [en]

Objective: To determine a predictive factor for the risk of conversion from ocular myasthenia gravis (OMG) to generalized MG (GMG) in a prospective study.

Methods: RNA was isolated from serum samples and detection of microRNA (miRNA) expression analyzed with qPCR. In the discovery set, 179 human miRNAs were assayed for profiling of five OMG patients and four age- and gender-matched healthy controls. Based on the specific accumulation pattern of 19 miRNAs from the discovery set, in addition to miRNAs previously found elevated in generalized MG (GMG; miR-150-5p and miR-30e-5p), 21 miRNAs were subsequently analyzed in a validation cohort of 83 OMG patients (82 immunosuppression treatment naive; 49 male) within 3 months of diagnosis and at a follow-up visit (median duration 28 months from first visit).

Results: Thirteen patients generalized 14.8 +/- 12.0 months after the diagnosis and the majority (85%) belonged to the late onset MG group. Two miRNAs were significantly higher in secondary GMG (SGMG) patients compared to OMG patients with late onset MG: miR-30e-5p (9.1 +/- 0.5 vs. 6.3 +/- 0.9; P < 0.0001) and miR-150-5p (7.4 +/- 1.1 vs. 6.4 +/- 1.1; P = 0.01). The sensitivity for miR-30e-5p in differentiating OMG and SGMG was 96% in all OMG patients and 100% in late onset OMG patients.

Interpretation: This is the first study to describe a potential predictive factor associated with the risk of generalization for patients with OMG. Raised levels (>8) of miR-30e-5p at initial presentation in patients with ocular MG symptoms, give a predictive cut-off for subsequent generalization of 96-100%.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 6, no 2, p. 243-251
National Category
Neurology
Identifiers
URN: urn:nbn:se:uu:diva-379273DOI: 10.1002/acn3.692ISI: 000459708100004PubMedID: 30847357OAI: oai:DiVA.org:uu-379273DiVA, id: diva2:1296820
Funder
Swedish Research Council, VR-523-2014-2048Available from: 2019-03-18 Created: 2019-03-18 Last updated: 2019-03-18Bibliographically approved

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