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Extracellular matrix mimetics by crosslinked peptide hydrogels: application to neural 3D cell cultures
Ferentis UAB, Savanorių 231, LT-02300, Vilnius, Lithuania; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanorių 231, LT-02300, Vilnius, Lithuania.
Ferentis UAB, Savanorių 231, LT-02300, Vilnius, Lithuania; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanorių 231, LT-02300, Vilnius, Lithuania.
Ferentis UAB, Savanorių 231, LT-02300, Vilnius, Lithuania; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanorių 231, LT-02300, Vilnius, Lithuania.
Ferentis UAB, Savanorių 231, LT-02300, Vilnius, Lithuania.
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2018 (English)In: International conference Vita Scientia Conference book, 2018, p. 59-59Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Self-supporting, shapeable hydrogels that consist of self-assembling synthetic peptides mimic the structural blocks of the extracellular matrix (ECM). Although they have been developed for regenerative medicine purposes, with a potential of grafting into patients without transplantation from organ donors, this class of materials are attractive as scaffolds for advanced cell culture/ in vitro tissue applications. In the present study, we have combined a series of peptides with functional motives (collagen, fibronectin, and laminin-like) for promoting granule layer-like organization of primary cerebellar cells and for controlling the cell attachment, neuritogenesis, cluster size and organization. We show that the micro/nanofabricated hydrogel scaffolds are applicable as multiwell plate inserts helping to analyse cell migration, differentiation, proliferation, adhesion, ultimately forming organotypic cell culture and artificial tissue structures.

Place, publisher, year, edition, pages
2018. p. 59-59
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-374371OAI: oai:DiVA.org:uu-374371DiVA, id: diva2:1280808
Conference
International conference: Vita Scientia. Vilnius, January 3, 2018
Available from: 2019-01-21 Created: 2019-01-21 Last updated: 2019-01-21Bibliographically approved

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fulltext(2333 kB)14 downloads
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Type fulltextMimetype application/pdf

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