Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Energy Landscape Analysis of the Full-Length SAM-I Riboswitch using Single-Molecule FRET Spectroscopy
Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry. Heidelberg University, Heidelberg, Germany.
Freie Universität Berlin, Berlin, Germany.
Show others and affiliations
2018 (English)In: Biophysical Journal, 2018, Vol. 114Conference paper, Published paper (Refereed)
Abstract [en]

Binding of the ligand S-adenosyl-L-methionine (SAM) produces major structural changes in the SAM-I riboswitch (RS) and thereby regulates gene expression via transcription termination. As yet, the conformations and motions governing the function of the full-length Bacillus subtilis yitJ SAM-I RS have not been deeply investigated. We have studied its conformational energy landscape as a function of Mg2+ and SAM ligand concentrations using single-molecule Förster resonance energy transfer (smFRET) microscopy. smFRET histograms of differently FRET-labeled constructs were so complicated that they could only be resolved with the help of kinetic experiments on immobilized riboswitches and hidden Markov modeling (HMM) analysis. At least four conformational states were identified, both in the presence and the absence of SAM. We determined their Mg2+-dependent fractional populations and conformational dynamics, including state lifetimes, interconversion rate coefficients and equilibration timescales. Riboswitches with terminator and antiterminator folds were found to coexist under all conditions; SAM binding induced only a gradual increase in the population of terminator states. Conformational transitions were much faster with bound SAM, which may be crucial for off-switching during the brief decision window before expression of the downstream gene.

Place, publisher, year, edition, pages
2018. Vol. 114
Series
Biophysical journal
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-374202DOI: 10.1016/j.bpj.2017.11.3692OAI: oai:DiVA.org:uu-374202DiVA, id: diva2:1280368
Conference
Biophysical Society
Available from: 2019-01-18 Created: 2019-01-18 Last updated: 2019-09-25Bibliographically approved

Open Access in DiVA

fulltext(67 kB)66 downloads
File information
File name FULLTEXT01.pdfFile size 67 kBChecksum SHA-512
2106deecf7d79aceb613205f6c32e616b89fc82ac50c8198af39290898a7750d3ddd7ee6e5aa59655f4e3901657062a6085a76d4f2b0dc9aefa24ef0c3321759
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Samanta, Ayan
By organisation
Polymer Chemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 66 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 69 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf