Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Fabrication and characterization of a blood-brain barrier on-a-chip for electrical characterization of cells
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. (EMBLA)
2018 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The blood-brain barrier (BBB) is crucial to maintain brainhomeostasis and prevent toxic substances from entering the brain.Endothelial cells (EC) are essential for the BBB and in this thesistwo different BBB-on-chips were designed for electricalcharacterization of immortalized mouse EC (bEnd3).Indium-Tin-Oxide (ITO) coated glass slides were etched, creating ITOelectrodes with increasing distance between them. The glass slideswere attached to a 3D-printed plastic well with UV-glue.

The second prototype was an extension of the first prototype with acopper printed circuit board (PCB) attached to the ITO glass slidesusing silver epoxy to connect the ITO electrodes to the copperelectrodes. The aim with these two chips was to create chips withtransparent electrodes for live imaging of the cells with an opticalmicroscope.

The chips were characterized with scanning electron microscopy (SEM) and a profilometer beforeseeding the cells inside the well. The absolute impedance wasmeasured across two parallel electrodes at a time. The impedance wasplotted against the distance between the electrodes. The method usedis called transmission line measurements (TLM) and is used to extractthe sheet impedance between the electrodes to evaluate the barriertightness of the cells.

Only one chip from each prototype remained intact after thefabrication and sterilization, making it difficult to drawconclusions from the impedance measurements. However, based on thetwo chips, the TLM for the first prototype followed a linear trendwith a high R-square value whereas, the second prototype showed largevariations, causing the R-square value to decrease

Place, publisher, year, edition, pages
2018. , p. 32
Series
UPTEC K, ISSN 1650-8297 ; 18034
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-369978OAI: oai:DiVA.org:uu-369978DiVA, id: diva2:1271789
Subject / course
Material
Educational program
Master Programme in Chemical Engineering
Supervisors
Examiners
Available from: 2019-02-01 Created: 2018-12-18 Last updated: 2019-02-01Bibliographically approved

Open Access in DiVA

fulltext(2375 kB)70 downloads
File information
File name FULLTEXT01.pdfFile size 2375 kBChecksum SHA-512
a1c424a0294bf55e00acab010f12766bcc120b053a5707171ef1332adf25a272f5885c948ca1f59ae5950c195388a150e13c1bc8ee735d65a7ffa54b20042745
Type fulltextMimetype application/pdf

By organisation
Microsystems Technology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 70 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 135 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf