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Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain: molecular characterization
Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain.
Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain;Biomed Res Ctr Network Resp Dis CIBERES, San Sebastian, Spain.
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2018 (English)In: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 24, no 8, p. 910.e5-910.e8Article in journal (Refereed) Published
Abstract [en]

Objectives: Chlamydia trachomatis ompA genotypes A and B, primarily associated with trachoma, were unexpectedly detected in urogenital samples of patients in Spain, a trachoma-free country. In this study, we aimed to explain this finding using analysis of organotropism-related genes and a multilocus sequence typing (MLST) technique.

Methods: C trachomatis genotypes A or B were detected in 8/930 (0.9%) infection episodes between 2006 and 2012. In these strains, organotropism-related genes (polymorphic membrane protein gene H, tryptophan synthase gene A, CTA0934, and cytotoxin) were studied. Further, the strains were analysed by MLST, using a polymerase chain reaction that amplifies five highly variable genomic loci (hctB, CT058, CT144, CT172, and pbpB). Amplicons were sequenced and phylogenetic analysis was conducted.

Results: Seven strains were detected in the eight infection episodes (in one patient, an identical strain being found in two episodes). Analysis of organotropism-related genes showed that these strains shared genetic features characteristic of genitotropic genotypes but not of trachoma strains. Three strains of genotype A showed a unique and new MLST-sequence type (ST551, allele profile 8-8-2-27-69). The four strains of genotype B belonged to ST138.

Conclusions: C. trachomatis ompA genotypes A and B associated with trachoma, but detected sporadically in urogenital samples in trachoma-free countries, may be the result of recombination between strains adapted to trachoma and strains adapted to sexual transmission.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2018. Vol. 24, no 8, p. 910.e5-910.e8
Keywords [en]
Chlamydia trachomatis, Multilocus sequence typing, ompA genotypes A and B, Organotropic genes, Urogenital samples
National Category
Microbiology in the medical area Microbiology
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URN: urn:nbn:se:uu:diva-361495DOI: 10.1016/j.cmi.2018.01.025ISI: 000439403900024PubMedID: 29427803OAI: oai:DiVA.org:uu-361495DiVA, id: diva2:1251226
Available from: 2018-09-26 Created: 2018-09-26 Last updated: 2018-09-26Bibliographically approved

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