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Using fluorescent probes and FRAP to investigate macromolecule diffusion in steam-exploded wood
Chalmers University of Technology, Sweden.
RISE - Research Institutes of Sweden, Bioscience and Materials, Agrifood and Bioscience. Chalmers University of Technology, Sweden.
RISE - Research Institutes of Sweden, Bioscience and Materials, Agrifood and Bioscience. Chalmers University of Technology, Sweden.ORCID iD: 0000-0001-9979-5488
Chalmers University of Technology, Sweden.
2018 (English)In: Wood Science and Technology, ISSN 0043-7719, E-ISSN 1432-5225Article in journal (Refereed) In press
Abstract [en]

Diffusion of fluorescently labeled dextran of varying molecular weight in wood pretreated by steam explosion was studied with a confocal microscope. The steam explosion experiments were conducted at relatively mild conditions relevant for materials biorefinery at a pressure of 14 bars for 10 min. The method of fluorescence recovery after photobleaching (FRAP) was used to perform diffusion measurements locally in the wood microstructure. It was found that the FRAP methodology can be used to observe differences in the diffusion coefficient based on localization in the microstructure, i.e., earlywood, latewood, and cell wall. Microscopic changes due to steam explosion were seen to increase diffusion of the smaller 3-kDa dextran diffusion probe in the earlywood, while the latewood structure was not affected in any significant way. Macroscopic changes to the structure in the form of ruptures due to the steam explosion pretreatment were observed to increase the rate of diffusion for the larger 40-kDa dextran probe.

Place, publisher, year, edition, pages
2018.
Keywords [en]
Dextran, Explosions, Fluorescence, Microstructure, Photobleaching, Probes, Steam, Steam engineering, Biorefineries, Cell walls, Diffusion measurements, Earlywood, Fluorescence recovery after photobleaching, Fluorescent probes, Pre-Treatment, Steam explosion, Diffusion
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-34478DOI: 10.1007/s00226-018-1039-5Scopus ID: 2-s2.0-85050675818OAI: oai:DiVA.org:ri-34478DiVA, id: diva2:1237308
Available from: 2018-08-08 Created: 2018-08-08 Last updated: 2018-08-15Bibliographically approved

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