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A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis
KTH Royal Inst Technol, Sci Life Lab, Div Prote & Nanobiotechnol, Stockholm, Sweden.
KTH Royal Inst Technol, Sci Life Lab, Div Prote & Nanobiotechnol, Stockholm, Sweden.
Karolinska Inst, Dept Publ Hlth Sci, Stockholm, Sweden;South Gen Hosp, Sachs Children & Youth Hosp, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
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2018 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 183, p. 192-200Article in journal (Refereed) Published
Abstract [en]

Paper-based biosensors offer a promising technology to be used at the point of care, enabled by good performance, convenience and low-cost. In this article, we describe a colorimetric vertical-flow DNA microarray (DNAVFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Different assay parameters such as hybridization buffer, flow rate, printing buffer and capture probe concentration were optimized. A limit of detection (LOD) of 4.4 nM was achieved as determined by tabletop scanning. The DNA-VFM was applied as a proof of concept for detection of Neisseria meningitidis, a primary cause of bacterial meningitis. The LOD was determined to be between 38 and 2.1 x 10(6) copies/VFMassay, depending on the choice of DNA capture probes. The presented approach provides multiplex capabilities of DNA microarrays in a paper-based format for future point-of-care applications.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 183, p. 192-200
Keywords [en]
Microarray, Paper-based biosensor, Point-of-care, Gold nanoparticles, Neisseria meningitidis
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-356185DOI: 10.1016/j.talanta.2018.02.070ISI: 000430645800026PubMedID: 29567164OAI: oai:DiVA.org:uu-356185DiVA, id: diva2:1236514
Funder
Swedish Research CouncilEU, European Research Council, 615458Available from: 2018-08-02 Created: 2018-08-02 Last updated: 2018-08-02Bibliographically approved

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