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Microbial rRNA gene expression and co-occurrence profiles associate with biokinetics and elemental composition in full-scale anaerobic digesters
Department of Civil Engineering, The University of British Columbia, Vancouver, BC, Canada.
Linköping University, Department of Thematic Studies, Tema Environmental Change. Linköping University, Faculty of Arts and Sciences. Linköping University, Biogas Research Center.
Linköping University, Department of Management and Engineering, Environmental Technology and Management. Linköping University, Faculty of Arts and Sciences.
Linköping University, Department of Thematic Studies, Tema Environmental Change. Linköping University, Faculty of Arts and Sciences. Linköping University, Biogas Research Center.
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2018 (English)In: Microbial Biotechnology, ISSN 1751-7907, E-ISSN 1751-7915, Vol. 11, no 4, p. 694-709Article in journal (Refereed) Published
Abstract [en]

This study examined whether the abundance and expression of microbial 16S rRNA genes were associated with elemental concentrations and substrate conversion biokinetics in 20 full-scale anaerobic digesters, including seven municipal sewage sludge (SS) digesters and 13 industrial codigesters. SS digester contents had higher methane production rates from acetate, propionate and phenyl acetate compared to industrial codigesters. SS digesters and industrial codigesters were distinctly clustered based on their elemental concentrations, with higher concentrations of NH3-N, Cl, K and Na observed in codigesters. Amplicon sequencing of 16S rRNA genes and reverse-transcribed 16S rRNA revealed divergent grouping of microbial communities between mesophilic SS digesters, mesophilic codigesters and thermophilic digesters. Higher intradigester distances between Archaea 16S rRNA and rRNA gene profiles were observed in mesophilic codigesters, which also had the lowest acetate utilization biokinetics. Constrained ordination showed that microbial rRNA and rRNA gene profiles were significantly associated with maximum methane production rates from acetate, propionate, oleate and phenyl acetate, as well as concentrations of NH3-N, Fe, S, Mo and Ni. A co-occurrence network of rRNA gene expression confirmed the three main clusters of anaerobic digester communities based on active populations. Syntrophic and methanogenic taxa were highly represented within the subnetworks, indicating that obligate energy-sharing partnerships play critical roles in stabilizing the digester microbiome. Overall, these results provide new evidence showing that different feed substrates associate with different micronutrient compositions in anaerobic digesters, which in turn may influence microbial abundance, activity and function.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2018. Vol. 11, no 4, p. 694-709
National Category
Microbiology
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URN: urn:nbn:se:liu:diva-149709DOI: 10.1111/1751-7915.13264ISI: 000435946800011PubMedID: 29633555Scopus ID: 2-s2.0-85045737255OAI: oai:DiVA.org:liu-149709DiVA, id: diva2:1234402
Note

Funding Agencies|Swedish Energy Agency; Biogas Research Centre at Linkoping University, Sweden; NSF [DGE-1256082]

Available from: 2018-07-24 Created: 2018-07-24 Last updated: 2018-08-14Bibliographically approved

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Svensson, Bo HSundberg, CarinaLarsson, MadeleineShakeri Yekta, Sepehr
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