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A model to study complement involvement in experimental retinal degeneration
Linnaeus Univ, Linnaeus Ctr Biomat Chem, Fac Hlth & Life Sci, Kalmar, Sweden.
Linnaeus Univ, Linnaeus Ctr Biomat Chem, Fac Hlth & Life Sci, Kalmar, Sweden.
Karolinska Inst, St Erik Eye Hosp, Sect Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology. Linnaeus Univ, Linnaeus Ctr Biomat Chem, Fac Hlth & Life Sci, Kalmar, Sweden.
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2018 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 123, no 1, p. 28-42Article in journal (Refereed) Published
Abstract [en]

Background: The complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system.

Method and materials: The adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19).

Results: Inflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture.

Discussion: Thus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD , 2018. Vol. 123, no 1, p. 28-42
Keywords [en]
AMD, complement system, ocular diseases, retina, RPE
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:uu:diva-354542DOI: 10.1080/03009734.2018.1431744ISI: 000428060300004PubMedID: 29436895OAI: oai:DiVA.org:uu-354542DiVA, id: diva2:1222549
Funder
Swedish Research CouncilStiftelsen Olle Engkvist ByggmästareMedical Research Council of Southeast Sweden (FORSS)Available from: 2018-06-21 Created: 2018-06-21 Last updated: 2018-06-21Bibliographically approved

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