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Etablering av MALDI-TOF MS och webbaserad referensdatabas för identifiering av dermatofyter och mögelsvamp
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2018 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Syftet med studien var att etablera Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry(MALDI-TOF MS) och onlinedatabasen Mass Spectrometry identification platform(MSI) som metod för identifiering av dermatofyter och mögelsvamp. Svamparna inkuberades 3 eller 6 dagar aerobt i 30°C på Sabouraud dextros agar. Två olika extraktionsmetoder användes innan analys med MALDI-TOF MS. Spektra analyserades med Flexcontrol version 3.4 och resulterade i scorevärde. De spektra som framkom skickades även till MSI för ytterligare analys där resultat erhölls som sannolikhet i procent. Av de ursprungliga 23 st prov identifierades totalt 22st(95,6%) efter 3 eller 6 dagars inkubation och med extraktionsmetod 1 eller 2. MSI databasen var mer omfattande än den interna databasen, men använde sig inte av de aktuella namnen. Behovet av artidentifiering kan ifrågasättas, då många dermatofyter och mögel inom samma släkte behandlas på samma sätt. Artbestämning för immunsupprimerade patienter kan däremot vara viktigt. Studien var lyckad och metoderna kommer tillämpas inom en snar framtid på Klinisk Mikrobiologi i Halmstad.

Abstract [en]

The purpose of this study was to establish Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry(MALDI-TOF MS) and the online database Mass Spectrometry identification platform(MSI) as a method for identification of dermatophytes and molds. The fungi were incubated 3 or 6 days aerobic in 30°C on Sabouraud dextrose agar. Two different extraction methods were used before analysis with MALDI-TOF MS. Spectra analyzed with Flexcontrol version 3.4 resulted in score values. The same spectra was later sent for analysis with MSI and the result obtained was seen as probability in percent. Of the original 23 samples a total of 22(95,6%) samples were identified after 3 or 6 days of incubation and by extraction method 1 or 2.  The MSI database was more comprehensive than the internal database, but did not use the current fungi names. The necessity of species identification can be questioned, since dermatophytes and molds within the same genera is treated the same way. Species identification for immunosuppressed patients could however be significant. The study was successful and the methods will be applied within a near future in the Clinical Microbiology laboratory in Halmstad.

Place, publisher, year, edition, pages
2018. , p. 23
Keywords [en]
Fungi, Ascomycetes, Protein extraction, Bruker, MSI
Keywords [sv]
Fungi, Ascomycetes, Proteinextraktion, Bruker, MSI
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:hj:diva-40343ISRN: JU-HHJ-BLA-1-20180065OAI: oai:DiVA.org:hj-40343DiVA, id: diva2:1220272
Subject / course
HHJ, Biomedical Laboratory Science
Available from: 2018-06-21 Created: 2018-06-18 Last updated: 2018-06-21Bibliographically approved

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CiteExportLink to record
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