Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Testing of micro-fluidic systems for Raman spectroscopic measurements on biological cells
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Pulmonary Artery Hypertension (PAH) is a condition that can affect people as a consequence of infections or diseases such as lung disease, high blood pressure or pneumonia. When afflicted by these diseases, low oxygen content in the lung tissue causes the pulmonary arterial soft muscle cells (PASMC) located in the walls of the pulmonary arteries to chronically swell up. As a result, the arteries are constantly narrowed. This can in many cases be fatal as the arteries become clogged and the heart is forced to pump more blood to the lungs, causing an enlargement of the right heart chamber which eventually may lead to heart failure. This irreversible swelling of the PASMC is the cause for PAH. To find a treatment for this incurable disease, the mechanisms of the vasoconstriction need to be investigated. 

Spectroscopy is the study of the interactions between light and matter and is a tool that can be used to gain knowledge in the matter of the expansions of the PASMC. In particular, Raman spectroscopy that targets the inelastic interactions can be used since it registers dynamic changes of cells. 

To simulate an oxygen deprived environment, a micro-fluidic system designed for use in cellular experiments has been developed. Tests of the prototypes showed strong Raman signals from the polymeric material of the system itself. These signals overshadowed the signals from the observed sample. The objective of the experiments presented in this report was to test whether the signals from the micro-fluidic system could be eliminated by adding spacing between the polymer and the sample. 

The experiment was conducted by collecting data of samples from baker's yeast prepared in the micro-fluidic system at different z-distances. By this the optimal spacing between the polymer of the micro-fluidic system and the sample could be determined. This experiment concluded that the sample needed to be placed 1.54 mm further from the micro-fluidic system in order to test human lung tissue at 2 mW laser intensity.

Place, publisher, year, edition, pages
2018.
National Category
Other Physics Topics
Identifiers
URN: urn:nbn:se:ltu:diva-69582OAI: oai:DiVA.org:ltu-69582DiVA, id: diva2:1219147
Subject / course
Student thesis, at least 15 credits
Educational program
Engineering Physics and Electrical Engineering, bachelor's level
Supervisors
Examiners
Available from: 2018-06-25 Created: 2018-06-15 Last updated: 2018-06-25Bibliographically approved

Open Access in DiVA

fulltext(2574 kB)4 downloads
File information
File name FULLTEXT01.pdfFile size 2574 kBChecksum SHA-512
a8de04330d2db84bce5c656525191f9af76529a7300d91237f75ad439f6e3ce162fcd41f2d80569648b19a480c43d8e85c01804c59dcbbd704d5eaf3e82a5755
Type fulltextMimetype application/pdf

By organisation
Department of Computer Science, Electrical and Space Engineering
Other Physics Topics

Search outside of DiVA

GoogleGoogle Scholar
Total: 4 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 43 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf