Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The discolouration of Hyaluronan in presence of phosphate buffer
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Show others and affiliations
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Hyaluronan is a polymer that among other things is used in fillers. Products containing Hyaluronan is sometimes discoloured over time and the mechanism behind this discolouration is still unknown. However, it was suspected that discolouration occurs during the degradation due to high pH values or with a phosphate buffer. The discolouration of Hyaluronan that occurs with phosphate buffer was studied in more detail in this project. The samples of Hyaluronan with different concentrations of phosphate buffer were left at 90 °C in an oven to speed up the discolouration. These samples were then analyzed byusing UV/Vis spectrophotometry to measure the absorption and capillary viscometry to measure the molecular weight. The results showed that the discolouration increased with time and that the samples with the higher concentration of buffer got more discoloured faster. The molecular weight showed a decreasing trend with time. It also suggested that the phosphate buffer had an impact on the molecular weight. The samples with the highest concentration of phosphate buffer had a lower molecular weight compared to samples with no phosphate buffer. The main conclusion from this study is that the phosphate buffer had an effect on the discolouration of Hyaluronan.

Place, publisher, year, edition, pages
2018. , p. 32
Series
TVE ; TVE-K 18 003
Keywords [en]
Hyaluronan, degradation, Molecular weight, yellow, discolouration
National Category
Polymer Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-353782OAI: oai:DiVA.org:uu-353782DiVA, id: diva2:1219131
External cooperation
Galderma
Educational program
Master Programme in Chemical Engineering
Supervisors
Examiners
Available from: 2018-06-20 Created: 2018-06-15 Last updated: 2018-06-20Bibliographically approved

Open Access in DiVA

fulltext(1455 kB)25 downloads
File information
File name FULLTEXT01.pdfFile size 1455 kBChecksum SHA-512
edcc5ed240f669cea462a922c2fd7aed5576670ee08548ed997122a896de3b0cc51c25f4e9b73d92d19027264403f9760f21e20607eb6cd7047cabc72585f175
Type fulltextMimetype application/pdf

By organisation
Department of Engineering Sciences
Polymer Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 25 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 66 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf