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Size-dependent genotoxicity of silver, gold and platinum nanoparticles studied using the mini-gel comet assay and micronucleus scoring with flow cytometry
Karolinska Inst, Inst Environm Med, Nobels Vag 13, S-17177 Stockholm, Sweden.;Masaryk Univ, RECETOX, Kamenice 753-5,Pavilon A29, CZ-62500 Brno, Czech Republic..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2145-3650
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2206-0082
Karolinska Inst, Inst Environm Med, Nobels Vag 13, S-17177 Stockholm, Sweden..
2018 (English)In: Mutagenesis, ISSN 0267-8357, E-ISSN 1464-3804, Vol. 33, no 1, p. 77-85Article in journal (Refereed) Published
Abstract [en]

Metallic nanoparticles (NPs) are promising nanomaterials used in different technological solutions as well as in consumer products. Silver (Ag), gold (Au) and platinum (Pt) represent three metallic NPs with current or suggested use in different applications. Pt is also used as vehicle exhaust catalyst leading to a possible exposure via inhalation. Despite their use, there is limited data on their genotoxic potential and possible size-dependent effects, particularly for Pt NPs. The aim of this study was to explore size-dependent genotoxicity of these NPs (5 and 50 nm) following exposure of human bronchial epithelial cells. We characterised the NPs and assessed the viability (Alamar blue assay), formation of DNA strand breaks (mini-gel comet assay) and induction of micronucleus (MN) analysed using flow cytometry (in vitro microflow kit). The results confirmed the primary size (5 and 50 nm) but showed agglomeration of all NPs in the serum free medium used. Slight reduced cell viability (tested up to 50 mu g/ml) was observed following exposure to the Ag NPs of both particle sizes as well as to the smallest (5 nm) Au NPs. Similarly, at non-cytotoxic concentrations, both 5 and 50 nm-sized Ag NPs, as well as 5 nm-sized Au NPs, increased DNA strand breaks whereas for Pt NPs only the 50 nm size caused a slight increase in DNA damage. No clear induction of MN was observed in any of the doses tested (up to 20 mu g/ml). Taken together, by using the comet assay our study shows DNA strand breaks induced by Ag NPs, without any obvious differences in size, whereas effects from Au and Pt NPs were size-dependent in the sense that the 5 nm-sized Au NPs and 50 nm-sized Pt NPs particles were active. No clear induction of MN was observed for the NPs.

Place, publisher, year, edition, pages
OXFORD UNIV PRESS , 2018. Vol. 33, no 1, p. 77-85
National Category
Pharmacology and Toxicology
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URN: urn:nbn:se:kth:diva-225329DOI: 10.1093/mutage/gex027ISI: 000426079100011Scopus ID: 2-s2.0-85042721477OAI: oai:DiVA.org:kth-225329DiVA, id: diva2:1194635
Note

QC 20180403

Available from: 2018-04-03 Created: 2018-04-03 Last updated: 2019-08-20Bibliographically approved

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