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6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp PCC 6803
Heinrich Heine Univ Dusseldorf, Cluster Excellence Plant Sci CEPLAS, Inst Synthet Microbiol, Univ Str 1, D-40225 Dusseldorf, Germany.;Beuth Univ Appl Sci, Dept Biotechnol, Seestr 64, D-13347 Berlin, Germany..
Univ Turku, Dept Biochem, FI-20014 Turku, Finland..
Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Schanzlestr 1, D-79104 Freiburg, Germany..
Univ Turku, Dept Biochem, FI-20014 Turku, Finland..
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2017 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 17, article id 229Article in journal (Refereed) Published
Abstract [en]

Background: The 6S RNA is a global transcriptional riboregulator, which is exceptionally widespread among most bacterial phyla. While its role is well-characterized in some heterotrophic bacteria, we subjected a cyanobacterial homolog to functional analysis, thereby extending the scope of 6S RNA action to the special challenges of photoautotrophic lifestyles.

Results: Physiological characterization of a 6S RNA deletion strain (Delta ssaA) demonstrates a delay in the recovery from nitrogen starvation. Significantly decelerated phycobilisome reassembly and glycogen degradation are accompanied with reduced photosynthetic activity compared to the wild type. Transcriptome profiling further revealed that predominantly genes encoding photosystem components, ATP synthase, phycobilisomes and ribosomal proteins were negatively affected in Delta ssaA. In vivo pull-down studies of the RNA polymerase complex indicated that the presence of 6S RNA promotes the recruitment of the cyanobacterial housekeeping s factor SigA, concurrently supporting dissociation of group 2 s factors during recovery from nitrogen starvation.

Conclusions: The combination of genetic, physiological and biochemical studies reveals the homologue of 6S RNA as an integral part of the cellular response of Synechocystis sp. PCC 6803 to changing nitrogen availability. According to these results, 6S RNA supports a rapid acclimation to changing nitrogen supply by accelerating the switch from group 2 s factors SigB, SigC and SigE to SigA-dependent transcription. We therefore introduce the cyanobacterial 6S RNA as a novel candidate regulator of RNA polymerase sigma factor recruitment in Synechocystis sp. PCC 6803. Further studies on mechanistic features of the postulated interaction should shed additional light on the complexity of transcriptional regulation in cyanobacteria.

Place, publisher, year, edition, pages
2017. Vol. 17, article id 229
Keywords [en]
Transcriptional regulation, Small RNA, s factor, RNA polymerase, Cyanobacteria
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-345061DOI: 10.1186/s12866-017-1137-9ISI: 000417545700001PubMedID: 29216826OAI: oai:DiVA.org:uu-345061DiVA, id: diva2:1188614
Funder
EU, FP7, Seventh Framework Programme, 323,987Available from: 2018-03-08 Created: 2018-03-08 Last updated: 2018-03-08Bibliographically approved

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