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Trimethylene carbonate-caprolactone conduit with poly-p-dioxanone microfilaments to promote regeneration after spinal cord injury
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
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2018 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 66, p. 177-191Article in journal (Refereed) Published
Abstract [en]

Spinal cord injury (SCI) is often associated with scarring and cavity formation and therefore bridging strategies are essential to provide a physical substrate for axonal regeneration. In this study we investigated the effects of a biodegradable conduit made from trimethylene carbonate and c-caprolactone (TC) containing poly-p-dioxanone microfilaments (PDO) with longitudinal grooves on regeneration after SCI in adult rats. In vitro studies demonstrated that different cell types including astrocytes, meningeal fibroblasts, Schwann cells and adult sensory dorsal root ganglia neurons can grow on the TC and PDO material. For in vivo experiments, the TC/PDO conduit was implanted into a small 2-3 mm long cavity in the C3-C4 cervical segments immediately after injury (acute SCI) or at 2-5 months after initial surgery (chronic SCI). At 8 weeks after implantation into acute SCI, numerous 5HT-positive descending raphaespinal axons and sensory CGRP-positive axons regenerated across the conduit and were often associated with PDO microfilaments and migrated host cells. Implantation into chronically injured SCI induced regeneration mainly of the sensory CGRP-positive axons. Although the conduit had no effect on the density of OX42-positive microglial cells when compared with SCI control, the activity of GFAP-positive astrocytes was reduced. The results suggest that a TC/PDO conduit can support axonal regeneration after acute and chronic SCI even without addition of exogenous glial or stem cells.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 66, p. 177-191
Keywords [en]
Synthetic conduit, Tissue engineering, Biomaterials, Spinal cord injury, Regeneration
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:umu:diva-145385DOI: 10.1016/j.actbio.2017.11.028ISI: 000424309200013PubMedID: 29174588OAI: oai:DiVA.org:umu-145385DiVA, id: diva2:1186858
Available from: 2018-03-01 Created: 2018-03-01 Last updated: 2018-06-09Bibliographically approved

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Novikova, Liudmila N.Kolar, Mallappa K.Kingham, Paul J.Wiberg, MikaelNovikov, Lev N.
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Department of Integrative Medical Biology (IMB)Department of Surgical and Perioperative Sciences
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