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A novel type of multiterminal motor endplate in human extraocular muscles
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.
2018 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 59, no 1, p. 539-548Article in journal (Refereed) Published
Abstract [en]

Purpose: To investigate the relation between type of motor endplate, acetylcholine receptor (AChR) subunit composition, and fiber types in human extraocular muscles (EOMs).

Methods: EOM samples collected from subjects aged 34 to 82 years were serially sectioned and processed for immunohistochemistry, with specific antibodies against different myosin heavy chain (MyHC) isoforms, neurofilament, synaptophysin, and adult epsilon (ε) and fetal gamma (γ) AChR subunits as well as α-bungarotoxin.

Results: A novel type of motor endplate consisting of large, multiterminal en plaque endings was found in human EOMs, in addition to the previously well-described single en plaque and multiple en grappe endplates. Such novel endplates were abundant but exclusively observed in myofibers lacking MyHC slow and fast IIa but containing MyHC extraocular (MyHCeom), isoforms. Multiple en grappe endings were found only in myofibers containing MyHC slow-tonic isoform and contained fetal γ AChR subunit. Adult ε and fetal γ AChR subunits, alone or combined, were found in the multiterminal endplates. Distinct AChR subunits were present in adjacent motor endplates of a given myofiber containing MyHCeom.

Conclusions: Human EOMs have a more complex innervation pattern than previously described, comprising also a novel type of multiterminal motor endplate present in myofibers containing MyHCeom. The heterogeneity in AChR subunit composition in a given myofiber suggests the possible presence of polyneuronal innervation in human EOMs.

Place, publisher, year, edition, pages
Rockville: The Association for Research in Vision and Ophthalmology , 2018. Vol. 59, no 1, p. 539-548
Keywords [en]
extraocular muscles, motor endplate, multiterminal motor endplate, en grappe, en plaque, polyneuronal innervation, myosin heavy chain, acetylcholine receptor subunit
National Category
Cell and Molecular Biology Ophthalmology
Research subject
Medical Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-145396DOI: 10.1167/iovs.17-22554ISI: 000425855900064PubMedID: 29372252OAI: oai:DiVA.org:umu-145396DiVA, id: diva2:1186769
Available from: 2018-03-01 Created: 2018-03-01 Last updated: 2018-06-09Bibliographically approved

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