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Structural and functional aspects of the interaction partners of the small heat-shock protein in Synechocystis
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry. University of Oxford, Oxford, UK.ORCID iD: 0000-0002-9804-5009
University of Massachusetts, Amherst, USA; Alorica Inc, Irvine, CA USA.
University of Massachusetts, Amherst, USA; Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ USA.
University of Oxford, Oxford, UK.
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2018 (English)In: Cell stress & chaperones (Print), ISSN 1355-8145, E-ISSN 1466-1268, Vol. 23, no 4, p. 723-732Article in journal (Refereed) Epub ahead of print
Abstract [en]

The canonical function of small heat-shock proteins (sHSPs) is to interact with proteins destabilized under conditions of cellular stress. While the breadth of interactions made by many sHSPs is well-known, there is currently little knowledge about what structural features of the interactors form the basis for their recognition. Here, we have identified 83 in vivo interactors of the sole sHSP in the cyanobacterium Synechocystis sp. PCC 6803, HSP16.6, reflective of stable associations with soluble proteins made under heat-shock conditions. By performing bioinformatic analyses on these interactors, we identify primary and secondary structural elements that are enriched relative to expectations from the cyanobacterial genome. In addition, by examining the Synechocystis interactors and comparing them with those identified to bind sHSPs in other prokaryotes, we show that sHSPs associate with specific proteins and biological processes. Our data are therefore consistent with a picture of sHSPs being broadly specific molecular chaperones that act to protect multiple cellular pathways.

Place, publisher, year, edition, pages
2018. Vol. 23, no 4, p. 723-732
Keywords [en]
Bioinformatics, Cyanobacteria, Molecular chaperone, Small heat-shock protein, α-Crystallins
National Category
Bioinformatics and Systems Biology
Research subject
Bioinformatics
Identifiers
URN: urn:nbn:se:uu:diva-343347DOI: 10.1007/s12192-018-0884-3ISI: 000438691200025PubMedID: 29476342OAI: oai:DiVA.org:uu-343347DiVA, id: diva2:1185914
Funder
Swedish Research Council, 2015-00559EU, FP7, Seventh Framework Programme, 2015-00559
Note

Correction in: Cell Stress and Chaperones, 2018, vol. 23, issue 4, page 733

DOI: 10.1007/s12192-018-0901-6

Available from: 2018-02-26 Created: 2018-02-26 Last updated: 2018-11-27Bibliographically approved

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