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Combined transcriptome and translatome analyses reveal a role for tryptophan-dependent auxin biosynthesis in the control of DOG1-dependent seed dormancy
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Molecular Plant Physiology, Institute of Environmental Biology, Utrecht University, 3584 CH Utrecht, the Netherlands.ORCID iD: 0000-0002-5605-7984
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2018 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 217, no 3, p. 1077-1085Article in journal (Refereed) Published
Abstract [en]

The importance of translational regulation during Arabidopsis seed germination has been shown previously. Here the role of transcriptional and translational regulation during seed imbibition of the very dormant DELAY OF GERMINATION 1 (DOG1) near-isogenic line was investigated. Polysome profiling was performed on dormant and after-ripened seeds imbibed for 6 and 24 h in water and in the transcription inhibitor cordycepin. Transcriptome and translatome changes were investigated. Ribosomal profiles of after-ripened seeds imbibed in cordycepin mimic those of dormant seeds. The polysome occupancy of mRNA species is not affected by germination inhibition, either as a result of seed dormancy or as a result of cordycepin treatment, indicating the importance of the regulation of transcript abundance. The expression of auxin metabolism genes is discriminative during the imbibition of after-ripened and dormant seeds, which is confirmed by altered concentrations of indole-3-acetic acid conjugates and precursors.

Place, publisher, year, edition, pages
WILEY , 2018. Vol. 217, no 3, p. 1077-1085
Keywords [en]
Arabidopsis thaliana, auxin, polysome profiling, ribosome, seed dormancy, seed germination, anscription
National Category
Botany Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-145161DOI: 10.1111/nph.14885ISI: 000424541600015PubMedID: 29139127OAI: oai:DiVA.org:umu-145161DiVA, id: diva2:1185907
Available from: 2018-02-26 Created: 2018-02-26 Last updated: 2018-06-09Bibliographically approved

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