Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Determinism and Contingency Shape Metabolic Complementation in an Endosymbiotic Consortium
Univ Complutense Madrid, Fac Ciencias Quim, Dept Bioquim & Biol Mol 1, Madrid, Spain..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-4940-719X
Univ Complutense Madrid, Fac Ciencias Quim, Dept Bioquim & Biol Mol 1, Madrid, Spain..
Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA..ORCID iD: 0000-0002-6263-8021
Show others and affiliations
2017 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 8, article id 2290Article in journal (Refereed) Published
Abstract [en]

Bacterial endosymbionts and their insect hosts establish an intimate metabolic relationship. Bacteria offer a variety of essential nutrients to their hosts, whereas insect cells provide the necessary sources of matter and energy to their tiny metabolic allies. These nutritional complementations sustain themselves on a diversity of metabolite exchanges between the cell host and the reduced yet highly specialized bacterial metabolism-which, for instance, overproduces a small set of essential amino acids and vitamins. A well-known case of metabolic complementation is provided by the cedar aphid Cinara cedri that harbors two co-primary endosymbionts, Buchnera aphidicola BCc and Ca. Serratia symbiotica SCc, and in which some metabolic pathways are partitioned between different partners. Here we present a genome-scale metabolic network (GEM) for the bacterial consortium from the cedar aphid iBSCc. The analysis of this GEM allows us the confirmation of cases of metabolic complementation previously described by genome analysis (i.e., tryptophan and biotin biosynthesis) and the redefinition of an event of metabolic pathway sharing between the two endosymbionts, namely the biosynthesis of tetrahydrofolate. In silico knock-out experiments with iBSCc showed that the consortium metabolism is a highly integrated yet fragile network. We also have explored the evolutionary pathways leading to the emergence of metabolic complementation between reduced metabolisms starting from individual, complete networks. Our results suggest that, during the establishment of metabolic complementation in endosymbionts, adaptive evolution is significant in the case of tryptophan biosynthesis, whereas vitamin production pathways seem to adopt suboptimal solutions.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2017. Vol. 8, article id 2290
Keywords [en]
endosymbiotic bacteria, cross-feeding, metabolic evolution, metabolic modeling, stoichiometric analysis
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-342804DOI: 10.3389/fmicb.2017.02290ISI: 000415915600001PubMedID: 29213256OAI: oai:DiVA.org:uu-342804DiVA, id: diva2:1185316
Funder
Knut and Alice Wallenberg Foundation, 2012.0075Available from: 2018-02-23 Created: 2018-02-23 Last updated: 2018-02-23Bibliographically approved

Open Access in DiVA

fulltext(1504 kB)3 downloads
File information
File name FULLTEXT01.pdfFile size 1504 kBChecksum SHA-512
3e5a53c432b706c3c4ed91dd67bc313194af7ca40ea920f0e63b343550f8d10eea6e25c29997dbcaba54a87ed7d0168d65a504815bd51a59af944efdc10bb769
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Tamarit, DanielMori, Matteo
By organisation
Molecular EvolutionScience for Life Laboratory, SciLifeLab
In the same journal
Frontiers in Microbiology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 3 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 9 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf