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PFOS induces proliferation, cell-cycle progression, and malignant phenotype in human breast epithelial cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2018 (English)In: Archives of Toxicology, ISSN 0340-5761, E-ISSN 1432-0738, Vol. 92, no 2, p. 705-716Article in journal (Refereed) Published
Abstract [en]

Perfluorooctanesulfonic acid (PFOS) is a synthetic fluorosurfactant widely used in the industry and a prominent environmental toxicant. PFOS is persistent, bioaccumulative, and toxic to mammalian species. Growing evidence suggests that PFOS has the potential to interfere with estrogen homeostasis, posing a risk of endocrine-disrupting effects. Recently, concerns about a potential link between PFOS and breast cancer have been raised, but the mechanisms underlying its actions as a potential carcinogen are unknown. By utilizing cell proliferation assays, flow cytometry, immunocytochemistry, and cell migration/invasion assays, we examined the potentially tumorigenic activity of PFOS (100 nM–1 mM) in MCF-10A breast cell line. The results showed that the growth of MCF-10A cells exposed to 1 and 10 µM PFOS was higher compared to that of the control. Mechanistic studies using 10 µM PFOS demonstrated that the compound promotes MCF-10A proliferation through accelerating G0/G1-to-S phase transition of the cell cycle after 24, 48, and 72 h of treatment. In addition, PFOS exposure increased CDK4 and decreased p27, p21, and p53 levels in the cells. Importantly, treatment with 10 µM PFOS for 72 h also stimulated MCF-10A cell migration and invasion, illustrating its capability to induce neoplastic transformation of human breast epithelial cells. Our experimental results suggest that exposure to low levels of PFOS might be a potential risk factor in human breast cancer initiation and development.

Place, publisher, year, edition, pages
2018. Vol. 92, no 2, p. 705-716
Keywords [en]
Breast cancer, Cell transformation, CDK4, p53, MCF-10A cells, Perfluorooctanesulfonic acid
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-342428DOI: 10.1007/s00204-017-2077-8ISI: 000425526000013PubMedID: 29063134OAI: oai:DiVA.org:uu-342428DiVA, id: diva2:1184255
Available from: 2018-02-20 Created: 2018-02-20 Last updated: 2018-03-29Bibliographically approved

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