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3D printed water-soluble scaffolds for rapid production of PDMS micro-fluidic flow chambers
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0001-5475-1422
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Science and Technology, Department of Physics.
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 3372Article in journal (Refereed) Published
Abstract [en]

We report a novel method for fabrication of three-dimensional (3D) biocompatible micro-fluidic flow chambers in polydimethylsiloxane (PDMS) by 3D-printing water-soluble polyvinyl alcohol (PVA) filaments as master scaffolds. The scaffolds are first embedded in the PDMS and later residue-free dissolved in water leaving an inscription of the scaffolds in the hardened PDMS. We demonstrate the strength of our method using a regular, cheap 3D printer, and evaluate the inscription process and the channels micro-fluidic properties using image analysis and digital holographic microscopy. Furthermore, we provide a protocol that allows for direct printing on coverslips and we show that flow chambers with a channel cross section down to 40 x 300 μm can be realized within 60 min. These flow channels are perfectly transparent, biocompatible and can be used for microscopic applications without further treatment. Our proposed protocols facilitate an easy, fast and adaptable production of micro-fluidic channel designs that are cost-effective, do not require specialized training and can be used for a variety of cell and bacterial assays. To help readers reproduce our micro-fluidic devices, we provide: full preparation protocols, 3D-printing CAD files for channel scaffolds and our custom-made molding device, 3D printer build-plate leveling instructions, and G-code.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018. Vol. 8, article id 3372
National Category
Other Materials Engineering Other Engineering and Technologies not elsewhere specified Other Physics Topics
Identifiers
URN: urn:nbn:se:umu:diva-144631DOI: 10.1038/s41598-018-21638-wISI: 000425500300044OAI: oai:DiVA.org:umu-144631DiVA, id: diva2:1181373
Funder
Swedish Research Council, 2013-5379The Kempe Foundations, JCK-1622Available from: 2018-02-08 Created: 2018-02-08 Last updated: 2018-06-09Bibliographically approved

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Dahlberg, TobiasStangner, TimHanqing, ZhangWiklund, KristerLundberg, PetterEdman, LudvigAndersson, Magnus
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