Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Perturbation of Wound Healing, Cytoskeletal Organization and Cellular Protein Networks during Hazara Virus Infection
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Divison of Neurobiology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden.
Show others and affiliations
2017 (English)In: Frontiers in cell and developmental biology, ISSN 2296-634X, Vol. 5, article id 98Article in journal (Refereed) Published
Abstract [en]

Normal epithelial and endothelial renewal and healing after bacterial and viral challenges are essential for homeostasis along the intestine and the blood and lymphatic vessels. We thus investigated whether and how virus affects migration of human epithelial cells and specifically how the nucleocapsid protein (N) modulates the cellular proteome and interactome using human Caco-2 cells in a wound-healing assay with Hazara virus as a model. Here, Hazara virus blocked cell migration in a dose- and time-dependent manner, disrupted the actin cytoskeleton and specifically reduced the expression of the IQ-motif-containing GTPase-activating protein 1 (IQGAP1) and water channel aquaporin 6 (AQP6) that regulate cytoskeletal organization, water homeostasis and vesicle communication. Moreover, in the Caco-2 cell proteome, we identified several distinct groups of molecules associating with N upon Hazara virus infection, being involved in the ensemble of important cellular processes, e.g., chaperone activity, metabolism, cellular defense against infections, cell morphology, and migration. These events do not only facilitate the virus life cycle, but they are also crucial for membrane and cytoskeleton dynamics, cellular self-renewal and wound healing, being so essential for body integrity and homeostasis.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2017. Vol. 5, article id 98
Keywords [en]
IQGAP1; aquaporin 6; cellular proteome; epithelial barrier; epithelial migration; homeostasis; virus infection
National Category
Cell Biology Microbiology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-144852DOI: 10.3389/fcell.2017.00098PubMedID: 29209610OAI: oai:DiVA.org:liu-144852DiVA, id: diva2:1180117
Available from: 2018-02-05 Created: 2018-02-05 Last updated: 2018-02-21

Open Access in DiVA

fulltext(6367 kB)26 downloads
File information
File name FULLTEXT01.pdfFile size 6367 kBChecksum SHA-512
de34a1c2b95e33c7b021b0e3283bd98625c04b4eba6e5c91690396a995cfe1414e475a09d83f04f0900d4d762986e8415f7a828d12661a5e1aaeda5ff9775433
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Molinas, AndreaTurkina, MariaMagnusson, Karl-EricVikström, Elena
By organisation
Division of Microbiology and Molecular MedicineFaculty of Medicine and Health SciencesDivison of Neurobiology
Cell BiologyMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 26 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 63 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf