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Characterization of promoter regions for design of a cell specific promoter
2017 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Global warming is one of the modern world’s biggest problems and renewable fuels

are needed to meet this challenge. Biohydrogen is one alternative of a renewable fuel

and can be produced from genetically modified cyanobacteria. Although the

production is not yet efficient enough to compete with fossil fuels on a commercial

level, it has potential. By gaining knowledge regarding pathways and regulatory

mechanisms in cyanobacteria, genetic tools can be built and further enhance the

production of biohydrogen. A synthetic promoter, which is active only in hydrogen

producing cells, heterocysts, is an example of a wanted genetic tool. Such a promoter

would make it possible to overexpress proteins essential for hydrogen production in

heterocysts and thereby increase the production of hydrogen. In this study, several

promoters have been designed and compared quantitatively on both transcriptional

and translational levels. The promoters were compared regarding their heterocyst

specific expression and their strength. By comparison of the designed promoters, all

with different contents, the essential promoter regions have been identified and two

well characterized and heterocyst specific promoter have been proposed.

Place, publisher, year, edition, pages
2017.
Series
UPTEC X ; 17 029
National Category
Natural Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-340861OAI: oai:DiVA.org:uu-340861DiVA: diva2:1180087
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2018-02-06 Created: 2018-02-04 Last updated: 2018-02-06Bibliographically approved

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Annala, Katja
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf