Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Alpha-actinin of the chlorarchiniophyte Bigelowiella natans
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0003-3044-1256
2018 (English)In: PeerJ, ISSN 2167-8359, E-ISSN 2167-8359, Vol. 6, article id e4288Article in journal (Refereed) Published
Abstract [en]

The genome of the chlorarchiniophyte Bigelowiella natans codes for a protein annotated as an α-actinin-like protein. Analysis of the primary sequence indicate that this protein has the same domain structure as other α-actinins, a N-terminal actin-binding domain and a C-terminal calmodulin-like domain. These two domains are connected by a short rod domain, albeit long enough to form a single spectrin repeat. To analyse the functional properties of this protein, the full-length protein as well as the separate domains were cloned and isolated. Characerisation showed that the protein is capable of cross-linking actin filaments into dense bundles, probably due to dimer formation. Similar to human α-actinin, calcium-binding occurs to the most N-terminal EF-hand motif in the calmodulin-like C-terminal domain. The results indicate that this Bigelowiella protein is a proper α-actinin, with all common characteristics of a typical α-actinin.

Place, publisher, year, edition, pages
2018. Vol. 6, article id e4288
Keywords [en]
Spectrin repeat, Actin-binding protein, Bigelowiella natans, Alpha-actinin, Calcium-binding protein
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-144484DOI: 10.7717/peerj.4288ISI: 000423148400005PubMedID: 29372122OAI: oai:DiVA.org:umu-144484DiVA, id: diva2:1180028
Funder
Carl Tryggers foundation , CTS 13:31Available from: 2018-02-04 Created: 2018-02-04 Last updated: 2018-06-09Bibliographically approved

Open Access in DiVA

fulltext(42269 kB)16 downloads
File information
File name FULLTEXT01.pdfFile size 42269 kBChecksum SHA-512
0a12645b62d48db987b955693d5e3e8d6e4c56c7f55fb7034b1abcfcb84bbdb447d572330cea5048a14db9abb0178f1c932beda5ef916d62742e3c66d839502b
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Backman, Lars
By organisation
Department of Chemistry
In the same journal
PeerJ
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 16 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 135 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf