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Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration
Karolinska Institute, Department of Cell and Molecular Biology.
Huazhong Agricultural University, College of Animal Science and Technology.
Karolinska Institute, Department of Cell and Molecular Biology; Francis Crick Institute.
Karolinska Institute, Department of Cell and Molecular Biology.
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2017 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 8, article id 2286Article in journal (Refereed) Published
Abstract [en]

Salamanders exhibit an extraordinary ability among vertebrates to regenerate complex body parts. However, scarce genomic resources have limited our understanding of regeneration in adult salamanders. Here, we present the ~20 Gb genome and transcriptome of the Iberian ribbed newt Pleurodeles waltl, a tractable species suitable for laboratory research. We find that embryonic stem cell-specific miRNAs mir-93b and mir-427/430/302, as well as Harbinger DNA transposons carrying the Myb-like proto-oncogene have expanded dramatically in the Pleurodeleswaltl genome and are co-expressed during limb regeneration. Moreover, we find that a family of salamander methyltransferases is expressed specifically in adult appendages. Using CRISPR/Cas9 technology to perturb transcription factors, we demonstrate that, unlike the axolotl, Pax3 is present and necessary for development and that contrary to mammals, muscle regeneration is normal without functional Pax7 gene. Our data provide a foundation for comparative genomic studies that generate models for the uneven distribution of regenerative capacities among vertebrates.

Place, publisher, year, edition, pages
2017. Vol. 8, article id 2286
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:uu:diva-339764DOI: 10.1038/s41467-017-01964-9ISI: 000418570700001PubMedID: 29273779OAI: oai:DiVA.org:uu-339764DiVA: diva2:1179955
Funder
NIH (National Institute of Health), F32GM117806EU, European Research CouncilSwedish Research CouncilWenner-Gren FoundationsSwedish Cancer Society
Available from: 2018-02-02 Created: 2018-02-02 Last updated: 2018-02-02Bibliographically approved

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Brito, GoncaloZamani, NedaSandberg, RickardGrabherr, ManfredAndersson, Björn
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