Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Multifunctional microfluidic chip for optical nanoprobe based RNA detection - application to Chronic Myeloid Leukemia
University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal.
University of Nova Lisboa, Portugal.
University of Nova Lisboa, Portugal.
Istanbul Technical University, Turkey.
Show others and affiliations
2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 381Article in journal (Refereed) Published
Abstract [en]

Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2018. Vol. 8, article id 381
National Category
Other Medical Engineering
Identifiers
URN: urn:nbn:se:liu:diva-144557DOI: 10.1038/s41598-017-18725-9ISI: 000419673100017PubMedID: 29321602OAI: oai:DiVA.org:liu-144557DiVA, id: diva2:1178288
Note

Funding Agencies|FEDER funds through the COMPETE Programme; FCT - Portuguese Foundation for Science and Technology [POCI-01-0145-FEDER-007688, UID/CTM/50025/2013]; project DISERTOX [PTDC/CTM-NAN/2912/2014]; Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO - FCT/MCTES [UID/Multi/04378/2013]; ERDF [POCI-01-0145-FEDER-007728, PD/BD/52211/2013]

Available from: 2018-01-29 Created: 2018-01-29 Last updated: 2018-02-21

Open Access in DiVA

fulltext(2374 kB)7 downloads
File information
File name FULLTEXT01.pdfFile size 2374 kBChecksum SHA-512
c5ae6dc2b15e63eb8d1d6c0ae84a9a5fcea4786499034ea02dca89d32d342ed688e656e28efd4ee9bdf060df653d0b5998b8eb150506598846d88bc34c23b980
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Bernacka-Wojcik, Iwona
By organisation
Physics and ElectronicsFaculty of Science & Engineering
In the same journal
Scientific Reports
Other Medical Engineering

Search outside of DiVA

GoogleGoogle Scholar
Total: 7 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 146 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf