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Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser
ASTAR, Inst Mol & Cell Biol, Biopolis, Singapore 138673, Singapore..
Natl Univ Singapore, Dept Phys, Singapore 117557, Singapore.;Natl Univ Singapore, Ctr BioImaging Sci, Singapore 117546, Singapore..
ASTAR, Inst Mol & Cell Biol, Biopolis, Singapore 138673, Singapore.;Natl Univ Singapore, Dept Biochem, Singapore 117597, Singapore..
ASTAR, Inst Mol & Cell Biol, Biopolis, Singapore 138673, Singapore..
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2017 (English)In: CYTOSKELETON, ISSN 1949-3584, Vol. 74, no 12, p. 472-481Article in journal (Refereed) Published
Abstract [en]

A major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F-actin can be flow-aligned to a disorientation of approximately 5 degrees. Using this XFEL-based technique, we determine that gelsolin amyloids are comprised of stacked -strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual -synuclein amyloids.

Place, publisher, year, edition, pages
WILEY , 2017. Vol. 74, no 12, p. 472-481
Keyword [en]
fiber diffraction, filament systems, XFEL
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-340293DOI: 10.1002/cm.21378ISI: 000417746200005PubMedID: 28574190OAI: oai:DiVA.org:uu-340293DiVA, id: diva2:1178220
Conference
International Symposium on Now in Actin, DEC, 2016, Nagoya Univ, Nagoya, JAPAN
Funder
Knut and Alice Wallenberg FoundationSwedish Research CouncilThe Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
Available from: 2018-01-29 Created: 2018-01-29 Last updated: 2018-01-29Bibliographically approved

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Larsson, MårtenHattne, JohanSeibert, MarvinForsyth, Trevor
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