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Vitamin and Amino Acid Auxotrophy in Anaerobic Consortia Operating under Methanogenic Conditions
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Uppsala University, Science for Life Laboratory, SciLifeLab.
University of Alberta, Edmonton, Canada; Singapore-MIT Alliance for Research and Technology, Leipzig, Germany.
University of Alberta, Edmonton, Canada.
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2017 (English)In: mSystems, E-ISSN 2379-5077, Vol. 2, no 5, article id e00038-17Article in journal (Refereed) Published
Abstract [en]

Syntrophy among Archaea and Bacteria facilitates the anaerobic degra- dation of organic compounds to CH4 and CO2 . Particularly during aliphatic and aro- matic hydrocarbon mineralization, as in the case of crude oil reservoirs and petroleum-contaminated sediments, metabolic interactions between obligate mutu- alistic microbial partners are of central importance. Using micromanipulation com- bined with shotgun metagenomic approaches, we describe the genomes of complex consortia within short-chain alkane-degrading cultures operating under methano- genic conditions. Metabolic reconstruction revealed that only a small fraction of genes in the metagenome-assembled genomes encode the capacity for fermenta- tion of alkanes facilitated by energy conservation linked to H2 metabolism. Instead, the presence of inferred lifestyles based on scavenging anabolic products and inter- mediate fermentation products derived from detrital biomass was a common fea- ture. Additionally, inferred auxotrophy for vitamins and amino acids suggests that the hydrocarbon-degrading microbial assemblages are structured and maintained by multiple interactions beyond the canonical H2 -producing and syntrophic alkane degrader-methanogen partnership. Compared to previous work, our report points to a higher order of complexity in microbial consortia engaged in anaerobic hydrocar- bon transformation. IMPORTANCE

Place, publisher, year, edition, pages
2017. Vol. 2, no 5, article id e00038-17
Keywords [en]
Black Queen hypothesis, metagenomics, petroleum, syntrophy M
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-339039DOI: 10.1128/mSystems.00038-17ISI: 000414004100006OAI: oai:DiVA.org:uu-339039DiVA, id: diva2:1174601
Funder
Swedish Research Council, 2012-4592; 2012-3892Swedish Foundation for Strategic Research , ICA010-015Swedish Research Council Formas, 2012-986Swedish National Infrastructure for Computing (SNIC), b2013274; b2011032Available from: 2018-01-16 Created: 2018-01-16 Last updated: 2018-02-01Bibliographically approved

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Hubalek, ValerieBuck, MoritzBertilsson, StefanEiler, Alexander
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