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Quantification of Biofilm Formation of Aggregatibacter actinomycetemcomitans with Various Genotype and Phenotype
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
2017 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

ABSTRACT

 

Biofilm is associated to many chronic infections among which periodontitis in one. Aggregatibacter actinomycetemcomitans is a related bacterium associated to aggressive periodontitis with early onset in younger individuals. In a previous study where bacterial samples were collected from adolescents in Ghana, a specific genotype of A. actinomycetemcomitans, serotype b, was found to be highly virulent and associated to the increased risk of developing attachment loss (AL ≥ 3 mm).

This study aimed at determining biofilm formation on 45 serotype b isolates of A. actinomycetemcomitans from the Ghana study and identifying possible correlations with virulence properties of the bacterium, as well as clinical data of the infected individual. The hypothesis was that the capacity to form biofilm is a factor that contributes to the pathogenic potential of A. actinomycetemcomitans.

The isolates were cultivated on plastic microplates, stained and biofilm formation quantified spectrofotometrically. An increased absorbance was considered as enhanced ability to form biofilm.

All isolates formed biofilm. High and medium levels of biofilm formation significantly correlated to AP-PCR type 1 positive bacteria (P = 0.02 and P = 0.01 respectively). No significant association was found to AL in the infected individuals.

Even though the capacity to form biofilm in the initial phase of colonization might be important, its role in individuals already infected with A. actinomycetemcomitans is of subordinate to its pathogenic potential.

Place, publisher, year, edition, pages
2017. , p. 16
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-143975OAI: oai:DiVA.org:umu-143975DiVA, id: diva2:1174533
Educational program
Dentistry Programme
Presentation
2017-10-12, Sal B, Tandläkarhögskolan, Umeå, 10:25 (Swedish)
Supervisors
Examiners
Available from: 2018-01-16 Created: 2018-01-15 Last updated: 2018-01-16Bibliographically approved

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