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Detection and molecular typing of Cryptosporidium in South African wastewater plants
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Statens veterinärmedicinska anstalt. (Karin Troell, SVA (MIK))
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Cryptosporidium is a protozoan parasite infecting the intestines of its hosts, leading to acute diarrheal disease. Out of 26 recognized species, 14 are known to infect humans. Of most importance, from a human perspective are Cryptosporidium parvum and Cryptosporidium hominis, of which the former is known to have zoonotic potential. Globally, cryptosporidiosis affect people with lowered immune status particularly hard; among children under five it is the most important parasitic cause of gastroenteritis. In the region of KwaZulu-Natal, on the east coast of South Africa, Cryptosporidium is considered endemic. Drinking water is frequently collected from river systems and as Cryptosporidium spp. can be transmitted via contaminated water, this may be one source of infection. Research on the species distribution is important for outbreak investigations and prevention efforts. In water and wastewater such speciation is commonly performed using immunomagnetic separation, an antibody dependent method. There is however a suspicion that these antibodies have less affinity to some species and hence contorts the detected species distribution. An alternative approach is therefore of interest.

 

In the present study, Cryptosporidium diversity in wastewater collected from four different wastewater treatment plants in KwaZulu-Natal, is evaluated with an optimized antibody-free workflow and a single cell platform. It was shown that the workflow is suitable for complex samples, such as wastewater. Furthermore, diversity was assessed with amplicon sequencing, revealing four different species and genotypes. Further modifications of the methods used could benefit the field of Cryptosporidium research, along with improving global health and preventing disease outbreaks.

Place, publisher, year, edition, pages
2017. , p. 40
Keywords [en]
Cryptosporidium, single-cell, antibody-free, FACS, Crypto, Wastewater, Durban, KwaZulu-Natal, miSeq, IMS, mink genotype, deer mouse genotype III
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-338855OAI: oai:DiVA.org:uu-338855DiVA, id: diva2:1173869
External cooperation
Thor-Axel Stenström, Durban University of Technology, South Africa
Educational program
Master Programme in Infection Biology
Presentation
2017-05-31, A7:111, Uppsala biomedicinska centrum BMC, Husargatan 3, Uppsala, 09:15 (English)
Supervisors
Examiners
Available from: 2018-03-06 Created: 2018-01-14 Last updated: 2018-03-06Bibliographically approved

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